المساهمة في الدراسة الفيتوكيميائية والنشاطية البيولوجية لنبات

Abstract

Rhantherium suaveolens was detailed in the study as a member of the Asteraceae family, and its various chemical properties and biological activities were evaluated for its potential use in alternative and complementary medicine. Here are the highlights of what was discussed and discovered in the study.

In this experiment, we prepared the methanolic extract, where in this experiment we prepared the methanolic extract in return we also recorded a yield of 20.68%, followed by chemical detection which showed the presence of various secondary metabolic compounds in the whole plant with varying values, tertiary terpenes, alkaloids, tannins, flavonoids and polyphenols with the absence of saponins, followed by quantification of polyphenols, flavonoids and tannins. The quantification of polyphenols, flavonoids and tannins in the methanolic extract showed the highest amount of polyphenols (g/mg 0.51 ± 34.84) and flavonoids (2.22 ± 0.075mg/g). while dragons were estimated at 4.09 ± 0.28 mg/g.).

In evaluating the antioxidant activity of the extract, we relied on the DPPH and FRAP methods, where it was observed that a significant antioxidant activity was recorded using the studied extract, as the inhibition rate of free radicals in the extract was recorded at values of mg/ml () 0.62 ± 0.002 =IC50 (for DPPH), while in the FRAP assay it was 58.37%. On the other hand, the anticoagulant activity was studied by performing the following coagulation tests (TP, TCK, Fibrinogéne), where the TP results were high levels estimated at 81.53% for the extract compared to the control, while the TCK results show (34.54s). Fibrinogène was g/l2.58, indicating that the extract is able to prolong the level of fibrinogen. The in vitro anti-inflammatory activity of the extract was evaluated by disrupting the secondary structure of egg albumin protein, and the results indicated that the extract possesses a protein denaturation rate reducing activity, where the extract possesses a protein denaturation rate reducing activity. Regarding the antimicrobial activity, the results showed significant activity against various tested strains, where the greatest activity was on Pseudomonas aeruginosa with an inhibition diameter of 27±0.57mm at concentration D100mg where statistical analysis showed no significant difference between this result and the inhibition obtained by the antibiotic GN 50, also the methanolic extract showed good results on Escherichia coli and Staphylocoque aureus strains, and the methanolic extract showed good results on Escherichia coli and Staphylococcus aureus.