Préparation de nanoparticules à base de Lagmi Et leurs activités biologiques

Abstract

The juice of date palm, extracted from the palm tree, is a natural substance rich in biologically active compounds, making it of great importance in nanotechnological applications. This study aimed to synthesize selenium nanoparticles and evaluate their biological activities using date palm sugars. After the work we carried out, we obtained a normal yield in all studied samples. From a technical standpoint, to characterize these nanomaterials and understand their properties, we utilized X-ray diffraction technique(XRD), which showed remarkable results. Consequently, the crystalline size of the zinc sample was 21 nm, selenium sample was 14 nm, and the zinc-selenium integration was 17 nm. Additionally, UV-visible(UV) spectroscopy results revealed that compounds in the samples fell within the same theoretical range: Zn (340 nm), Se (379 nm), and pure extract (409 nm). Conversely, infrared spectroscopy unveiled (FT-IR) the presence of hydrogen and carbon bonds in the samples, distinguishing the nanoparticles. Moreover, scanning electron microscopy(SEM) clarified the nano shape and types of atoms present, namely zinc selenite(ZnSe), selenium oxide(SeO2), and zinc oxide(ZnO). Furthermore, the results of biological activities demonstrated strong and effective distinction in activity against bacteria by eliminating four known strains, including E. Coli, Pseudomonas aeruginosa, Staphylococcus, and Bacillus subtilis. Additionally, strong activity against hemolysis was recorded with Zn-SeNPs at 81.41% and Se-NPs at 98.62%, indicating powerful antioxidant capability and effective protection against red blood cell breakdown. Another promising biological activity studied for selenium nanoparticles SeNPs included DPPH, FRAP, Beta-carotene, in addition to anti-inflammatory and anti-coagulant activity.