Algerian Journal of Biosciences

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Advances in research on the use of Brevundimonas spp. to improve crop and soil fertility and for soil bioremediation
(Université of Eloued-جامعة الوادي, 2023-06-30) Souad, Zaim; Ahmed Amine, Bekkar
Biofertilizers or biological fertilizers maintain soil fertility by fixing atmospheric nitrogen, solubilizing P and K, producing plant growth substances and antibiotics as well as biodegradation of organic matter in the soil that enriches the root rhizosphere. Microbial biofertilizers are eco-friendly and less expensive alternatives to chemical fertilizers. The key components of healthy soil are populations of plant growth promoting rhizobacteria (PGPR) which play multiple beneficial and ecological roles in the rhizosphere soil. PGPR colonizes rhizosphere or plant roots, resulting in phytostimulation, biofertilization and biocontrol either directly and/or indirectly. Another important role of PGPR is its ability to decontaminate soils through a process called soil bioremediation. Recently, the known rhizobacteria environmentally friendly biofertilizers for sustainable agriculture are those belonging to Brevundimonas spp., which play a significant role in improving crop production and soil health .
Antimicrobial And Antioxidant Activity Of Methanol Extract Of Echinophora Spinosa L. From Jijel, Algeria
(جامعة الوادي - University of Eloued, 2020-08-31) Ghadbane, Mouloud; Bounar, Rabaha; Rebbas, Khellaf
In this study, the antioxidant and antimicrobial activities of Echinophora spinosa were investigated. Antimicrobial activity of methanol extracts obtained from Echinophora spinosa was examined using the disc diffusion method. Antioxidant activity of the methanol extracts was examined using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test. Methanol extract of ripe fruits of E. spinosa showed highest total phenolic content (69.17 ± 1.2 μg GAE/mg extract) and the major flavonoid contents (12.122 ± 0.44 μg QE/mg extract) was found in leaves of the plant. In disk diffusion antimicrobial assay, E. spinosa manifested broad spectrum of activity. The largest capacity to neutralize DPPH radicals was found for ripe fruits methanol extract of E. spinosa plant. The results shows that the various parts of E. spinosa extracts promising antioxidant and antimicrobial activities have potential bioactivities due to high content of phenolic and flavonoid compounds.
Assessment of Marrubium vulgare hydro-alcoholic extract’s biological activities
(Université of Eloued-جامعة الوادي, 2023-06-30) Mulondo, Samuel; Kimbugwe, Muzamiru; Lassami, Afaf; Chelghoum, Hayet
Marrubium vulgare is a tall, robust herbaceous perennial plant originating from Asia and Mediterranean and currently distributed throughout North, South America, Europe, Mediterranean and west Asia. M.vulgare thrives in any type of the soil but it prefers light calcareous, dry soils where it is sunny and warm at an altitude between 1500 –2400 m. This plant is becoming increasingly important because it is currently cultivated in different countries to be used as a source of medicine and food flavors. Also,it has more than 54 different phytocompounds such as polyphenols, monoterpenes, diterpenes and essential oils. Marrubin was the first isolated diterpenes and it’s characterized as the chemotaxonomic marker for the genus Marrubium. According to different studies, these compounds are responsible for antioxidant, antimicrobial, antifungal, analgesic, and anti-inflammatory, wound healing and anthelmintic activities. Due to these biological activities, hydroalcoholic extracts of Marrubim vulgare have been exploited for their therapeutic nature by traditional healers to cure several illnesses in Algeria. This review is to assess the different studies of hydroalcoholic extracts of Marrubium vulgare for different biological activities.
Biological Control By Plant Growth Promoting Rhizobacteria
(جامعة الوادي - University of Eloued, 2020-12-31) ben arbia, radia; Bestami Merdia . Ben Malek, Rokaia; Fellan, Kheira; Benaissa, Asmaa
Plant Growth Promoter Rhizobacteria (PGPR) is soil bacteria that can live on, in or around plant tissue and promote plant growth by many mechanisms that include a biological control of plant pathogens. Indeed, PGPRs have a protective effect through several modes of action such as antagonism, competition, production of hydrolytic enzymes and biofilm formation. Moreover, the use of PGPRs as biocontrol agents is very harmonious with the environment and therefore represents a good alternative to the use of chemicals in agriculture. This review is presented as a general bibliographical synthesis on the different aspects of PGPRs and their biocontrol potential.
Biosynthesis of Silver Nanoparticles and Their Applications
(Université of Eloued-جامعة الوادي, 2023-06-30) Emmanuel Chile, Nleonu; Ilham, Ben Amor; Soumeia, Zeghoud; Hadia, Hemmami
Recent developments in nanotechnology and nanoscience have improved methods for treating, preventing, and diagnosing a wide range of illnesses in many parts of living beings. Silver nanoparticles (Ag NPs) are among the most significant and intriguing metallic nanoparticles employed in several biological applications. To create Ag NPs, biomolecules from diverse microbial species and plant components have been researched as possible agents. Due to their physical orientation characteristics, and small size, these Ag NPs are widely employed and are said to have an impact on the performance of any other material that comes into touch with them. In addition, straightforward biological, physical, and chemical methods may be used to create Ag NPs. Due to their enhanced responsiveness to environmentally friendly technology for quantifiable synthesis, several developed nations have seen significant growth in the biosynthesis of Ag NPs. The biological method, however, is the approach to preparation that is most in demand since it is quicker, safer, less expensive, and more environmentally friendly than other techniques. In addition, the importance of Ag NPs is extensively examined in light of their numerous bioapplications, including those for antifungal, anti-inflammatory, antibacterial, Antiviral activity, Catalytic Activity, and anticancer medicines.
Boulaares, ISlam. Characterization And Acute Toxicity Evaluation Of The Mgo Nanoparticles Synthesized From Aqueous Leaf Extract Of Ocimum Basilicum L
(جامعة الوادي - University of Eloued, 2020-08-31) Derouiche, Samir; Guemari, Imane Yousra; Boulaares, ISlam
The aim of this study was to prepare magnesium oxide nanoparticles (MgONPs) using aqueous leave extract of Ocimum basilicum L. and to evaluate their acute toxicity. The characteristics of biosynthesized MgO powder was analyzed by UV–Vis spectroscopy, scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The acute toxicity test of MgONPs was applied in Wistar albino rats with different concentration. Results showed that the broad bell-shaped spectrum band was obtained by UV–Vis spectroscopy indicates the formation of MgO. The SEM images provided further insight into the shape and size of MgO which to be ranging under 440 nm. Fourier transform infrared (FTIR) spectroscopy detected the vibration of the Mg─O bond that indicate the presence of magnesium oxide nanoparticles (MgO). In this study, the toxicity test showed no mortality or behavioral change in low dose of MgNPs (250 mg / kg b.w) but we observed that 50% of rats have died when treated with high dose of MgNPs (500 mg/kg b.w.). This study confirmed that aqueous extract of Ocimum basilicum L. has potential properties as biocatalyst for the biosynthesis of MgONPs without any toxicity under dose 250 mg/kg in rats.
Characterization of endospore-forming bacteria producing extracellular enzymes isolated from the Djurdjura Mountains in Algeria
(جامعة الوادي - University of Eloued, 2021-06-30) Merzouk, YAHIAOUI; Khelifa, BOUACEb; Katia-Louiza, ASMANI; Sondes, MECHRI; Hamdi, BENDIF; Mohammed, HARIRe; Bassem, JAOUADI
Biodiversity in mountains in Algeria appears scanty and has not been thoroughly investigated. However, the mountain soil has been shown as an almost entire reserve of novel enzymes with interesting properties for industrial and environmental applications. In the present study, thirty bacterial strains were isolated from the Djurdjura Mountains in Kabylia (Algeria) and were studied for their ability to produce enzymes to be possibly used in biotechnological processes such as amylase, caseinase, and chitinase. The characterization of these isolates was carried out using morphological, physiological, and biochemical characteristics. All the data obtained with regards to the phenotypical properties of the isolates, confirmed that the strains belonged to the Bacillus group. In addition, the 16S rRNA gene of the two retained strains KA15 and LK-DZ15 was also amplified and sequenced. Phylogenetic tree was, afterwards, constructed. The nucleotide sequences and blast analyses confirmed that the KA15 and LK-DZ15 strains were closely related to those of the Bacillus altitudinis (accession n°.: MK874318) and Paenibacillus timonensis (accession n°.: MK734103) strains. The presence of amylases, proteases, and chitinases in KA15 and LK-DZ15 isolates are an indicator of their pivotal application in a variety of biotechnological processes.
Citrullus colocynthis: source of biodiesel
(جامعة الوادي - University of Eloued, 2022-06-30) Asmaa, Benaissa
Citrullus colocynthis (L.). Schrad, is a member of the botanical family of Cucurbitaceae and is commonly called coloquinte or "desert gourd", Handhal (Arabic) or Alkad (Tamahaq). This plant is known for its high oil content; it is a source of biodiesel and a real substitute for conventional diesel. Biodiesel is defined as a chemical alteration of vegetable oil to match the characteristics of the engine. The transesterification reaction is the most common method for converting coloquinte oil into esters and glycerol. The purpose of this communication is to draw attention to the biodiesel produced by coloquinte, which has been widely described in the literature.
Comparative Chiral Separation of (RS)‐Propranolol Racemate by HPLC Using α-Glycoprotein and β-Cyclodextrin Stationary Phases.
(جامعة الوادي - University of Eloued, 2021-12-30) Derouicha, MATMOUR; Fatima Zohra, HADJADJ AOUL; Yassine, MERAD; Nazim, BELLIFA; Hassen, GUERMOUCHE
The most of the β-blockers are still clinically being sold as a racemic mixture despite the fact that their enantiomers show significant differences in the pharmacological effects and activities. This paper describes a comparative study of tow chiral separations of (RS)‐Propranolol racemate by HPLC using α-Glycoprotein (AGP) and β-Cyclodextrin (BCD) Stationary Phases. For the AGP separation, the column size was (150 mm X4 mm X 5 μm), the mobile phase composed of Propanol-2 and Ammonium acetate (0.5:99.5 v/v), at a flow rate of 0.9 mL/min and the detection by ultraviolet absorption at 225 nm. For the BCD separation, the column size was (200 mm X4 mm X 5 μm), the mobile phase composed of Acetonitrile: Ethanol: Acetic acid: Triethylamine (960: 33: 4: 3 v/v/v/v), at a flow rate of 1 mL/min and the detection by ultraviolet absorption at 225 nm. The retention time of SPropranolol and R-Propranolol with AGP separation was respectively: 7.25 min and 11.82 min while with the BCD separation 16.18 min and 18.50 min respectively. The racemate contains 50.46 % of S-Propranolol and 49.53 % of R-Propranolol with AGP separation while with BCD separation, it contains 50.43 % of S- enantiomer and 49.57 % of R-enantiomer. There is a similarity between the enantiomeric purity values and the enantiomeric excess values of tow separations, but the separation with AGP stationary phase is faster than with the BCD stationary phase. For a selective β-blocking use, it could be very interesting to encourage its production in its form enantiomerically pure wich is the S-enantiomer.
Comparative study of the antibacterial action of natural and commercial oils of Rosmarinus officinalis L
(جامعة الوادي - University of Eloued, 2022-12-30) lakhdar, Mazouz
The article tries to shed light on the antibacterial power of rosemary oils to see if there is a possibility of making drugs but also to compare this power with that existing in commercial oils of rosemary and to check if these formulas really contain oils rosemary. This study consisted of the extraction of the essential oil from the studied plant followed by preliminary phytochemical tests and then by TLC analyzes to determine exactly the different compounds that characterize the species and then by the antibacterial study on the plants diluted essential oils (synthesized and commercial). Phytochemical tests have shown that essential oils contain many chemicals and active ingredients that give rosemary a wide range of indications : The TLC of the raw extract shows stains, indicating the presence of flavone, hydroxyflavone, Dihydroxyflavone, flavanones, methoxyflavones and flavonols ; they are compounds of paramount importance in the therapeutic effects enjoyed by rosemary. The bacteriological study on essential oils (extract and commercial) by the process represented on the concentrated disk of essential oils shows good antibacterial activity on the fixed stem ; the zone of inhibition is of 08 and 06 mm for Escherischia. coli and 10.09 and 08 mm for Staphylococcus aureus and 17, 14, 13.11 mm for Klebsiela sp. Essential oils extracted from rosemary from Algeria will be of some economic interest in the Algerian pharmaceutical industry. The negative test of commercial rosemary essential oil compared to the positive tests of the rosemary extract (just 10 mm diameter at concentration D1 for Klebsiella. sp and zero results at all other concentrations with the other strains including those that reacted to the rosemary extract tested ) provides information on the counterfeiting that characterises this field.. The study confirms the interest we must bring to this plant, not only for its characteristics in the perfume industry, but especially for its undisputed medical virtues.
Comparative sunscreen and stability studies of shea butter from Nigeria
(جامعة الوادي - University of Eloued, 2022-12-30) Ibrahim Mohammed, Hadiza; Zainab, Mohammed; Oyi Rukayat, Avuashi; Ibrahim, Garba
Shea butter extracted from the nuts of Vitellaria paradoxa is utilized as food, medicine and a significant source of income especially, in rural communities. The butter is reported to vary in their physicochemical compositions which may affect the sunscreen and stability of it, thus, the need for the present study. Shea nuts were collected from Ngaski (A), Bosso (B) and Yamaltu-Deba (C) of northern Nigeria. The in-vitro sunscreen activity was determined using UV-spectrophotometer and the stability study using plastic, clear and amber bottles stored in different conditions. The shea butter samples showed high SPF values at 1.00 % with shea butter C having the highest value at 37.49 % while shea butter A had the least value at 25.17. All the butter had sunscreen values less than 1 % at 0.50%. Samples stored in plastic, colourless and amber bottles in the refrigerator had higher moisture content. In comparison, those stored at room temperature had lower moisture content, although samples in amber bottles stored in the refrigerator were found to have fewer peroxide values.
Découverte de niger (Guizotia abyssinica (L.f.) Cass. 1829 (Asteraceae) en Algérie et mise au point sur son utilisation
(جامعة الوادي - University of Eloued, 2022-06-30) Khellaf, Rebbas; Saliha, Benhissen; Djamel, Sarri; Abdelouahab, Belkassam; Rabah, Bounar
Guizotia abyssinica (L. f) Cass. (Asteraceae) est originaire d’Ethiopie. Cette astéracée a été découverte dans la région de Sétif et de M’Sila, dans le nord-est de l’Algérie. Des notes écologiques et d’utilisations alimentaires sont également fournies.
Diversity and distribution patterns of reptiles in the northern Algerian Sahara (Oued Souf, Taibet and Touggourt)
(جامعة الوادي - University of Eloued, 2021-12-30) Aicha, MOUANE; Abdel Karim, HARROUCH; Ismail, GHENNOUM; Makhlouf, SEKOUR
The present study was based on the observations and captures of reptiles in the Northern Algerian Sahara (2011-2013). The studied habitats were the erg (sand dunes), palm grove, reg, Sebkha, and urban sites. We have identified 30 species (20 Saurians, 9 Ophidians and one Testudines) these species are classified into two orders (Anura and Squamata), 12 families and 22 different genera. Among the identified species, 7 protected species in Algeria and 5 species endemic to the Mediterranean. A total of 93.3% (28 species) are assessed as Least Concern and one species was considered to be Data Deficient (Scincopus fasciatus). One species is listed as near threatened (Uromastix acanthinurus). The highest species richness was noticed in the palm groves with 19 species as well as the Shannon diversity index= 3.35. The evenness is at its highest in the sebkha and the urban site with 0.9 and 0.86. The correspondence factorial analysis showed that some species were characteristic of habitats such as Tarentola neglecta and Tarentola deserti which are a specific species of the urban sites and the palm groves, while Scincus scincus was a specific species of the Erg. The generalized linear model showed that the geographic coordinate, spatiotemporal factors and human activity have significant effect on the distribution of reptiles.
Ecodendrometric Parameters Of Some Medicinal Plants In Aleppo Pine Forests Of Northern Sétif (algeria)
(جامعة الوادي - University of Eloued, 2020-12-31) rabihat, eadaad; Bounar, Rabah; Ghadbane, Mouloud; Chermat, Sabah; Rebbas, Khellaf
The ecodendrological study, on 35 plots were sampled in the Alep pine formation of the Ain Hamda Tizi N’bechar forest at the rate of one plot per station and in each plot a Phytoecological floristic survey was carried out. Concerning the species of the floral procession of the pine forest, there are 19 medicinal species, belonging to 13 families and 20 genera. This medicinal potential must be protected and preserved; it is in this context that this study is based on the knowledge of the factors which influence the distribution and the distribution of these species within the framework of a good rational management in support of the local and sustainable development of the local population through valuation.
Ecology and behavioral response of Ruddy Shelduck (Tadorna ferruginea) in the wetland complex of Oued Righ valley (Algerian Sahara).
(جامعة الوادي - University of Eloued, 2021-06-30) Yassine, NOUIDJEM; Asma, ZOUBIRIa; Sakina, MEROUANIa; Fateh, MIMECHE
The wetland complex of Oued Righ valley accommodates a population of Ruddy Shelduck (Tadorna ferruginea) between 2012 and 2014, the total reached 80 individuals in the Lake Hamraia where the species wintering. The analysis of the diurnal time budget of wintering populations revealed the role of feeding area delivery for this species and they devoted their day time to feeding (51.2%), sleeping (18.8%), followed by swimming activity (6.3 %), and courtship activities with flying (2.03%) which often occurs after disturbances caused by human or by Marsh harrier (Circus aeruginosus). It is interesting to study the behavior of these ducks in Saharan bioclimatic area and follow the phenology and characteristics in order to preserve the biodiversity in this region.
Effect of water salinity stress on dates biometric and biochemical characteristics
(جامعة الوادي - University of Eloued, 2022-06-30) Krid, Keltoum
The problem of water irrigationin palm groves led us to conduct this study based on the comparison of the quality of dates Deglet Nour and Ghars from two farming systems in the region of El Oued according to thesaline water irrigation degree. Salt stress in El Ghout causes in both varieties a regression of biometric characteristics, pH, EC, water content and dry matter, while total sugars are high. The dates of the farm are more voluminous, less acidic, with high ash content, dry matter and high electrical conductivity. According to standards Algerian of date classification reveals that the characteristics of Deglet Nour dates of El Ghout are more damaged by increasing the water salinity. The two varieties of the farm have better characteristics than those of El'Ghout. It's also appears that saline water irrigation improves the sugar content of dates
Effects Of Extraction Methods On Total Polyphenols, Free Radical Scavenging And Antibacterial Activity Of Crude Extracts Of Cleome Arabica L. Growing In Oued Souf Region
(جامعة الوادي - University of Eloued, 2020-08-31) Chouikh, Atef; Rebiai, Abdelkrim; Aref, Mahdia; Heded, Mounira; Adjal, El Hadda; Alia, Fatma
This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian). The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus.
The Effects Of The Fertil Verde Fertilizer On The Growth And Yield Of Chili Pepper (capsicum Annuum L) In Southern Algeria
(جامعة الوادي - University of Eloued, 2020-08-31) Ghemam Amara, Djilani; Kherraz, Khaled; Alia, Zeid; Chamsa, Ahmed Khalifa; Laouedj, Hacene; Senoussi, Mohammad Mourad
A field experiment was conducted on a private farm in El Oued State of Algeria to study the efficiency of using Fertil Verde as a fertilizer by foliar spry on hot pepper seedlings. The treatment by Fertil Verde increased all growth. The increase was significant in the number of main and secondary stems, the size of the leaves, and the weight of the dry leaves. While, the weight of the wet leaves did not significantly increase. Moreover, the use of Fertil Verde has led to a significant increase in the yield qualities of the plant: the weight and shape of the fruits, fresh yield of the plant, the content of pigment, and the size of the plant. Thus, the combination of fertilizing is common and Fertil Verde is a promising low-cost option in the production of high yields.
Ethnobotanical investigation and Morphobiometric characterization of different cowpea seeds (vigna unguiculata subsp. unguiculata (L.) walp.) in the Hoggar region (Algerian Sahara): acquisition and future investment project for food security in Algeria.
(جامعة الوادي - University of Eloued, 2021-12-30) Aida, BASSEDDIK; Sihem, TELLAH
This survey was conducted to establish an inventory of cowpea culture in the arid region of southern Algeria in 2018. To this end, missions were organized to collect accessions, provide information on cultural practices and traditional knowledge related to this culture a semi-questionnaire The European Commission has set up a European Agricultural Information Society. The study found that among the farmers surveyed. This study showed that of the farmers surveyed, 70.02% were men and 29.07% were women. Farmers cultivate cowpea on small areas, its production is used only for own consumption and a small one is marketed in villages where the plant is grown, the farmers interviewed use three main parts: seeds, green pods and hay. After harvest, the rest of the plant (leaves and stems) is used to feed livestock (sheep and cattle). Cowpea cultivation is in danger of extinction, according to the majority of farmers surveyed, diseases and insect attacks are low (32%). In addition, low rainfall, electricity and labor shortages during manual harvesting appear to be the main production constraints reported by the farmers interviewed. While farmers are satisfied with the yields obtained. The results obtained by biometric morpho characterization showed a wide phenotypic variability of the seeds However, significant differences were detected in several morphological characters.
Ethnobotanical survey of herbal remedies traditionally used in ElHammadia (Southern region of the province of Bordj Bou Arreridj, Algeria)
(جامعة الوادي - University of Eloued, 2021-06-30) Hamdi, BENDIF; Mohammed, HARIR; Merzouk, YAHIAOUI; Nabila, SOUILAHd; Fatima Zohra, HECHAICHI; Mohamed, DjamelMIAR; Ifriqya, MEDILA
This work is devoted to the study of medicinal plants in the southern region of BordjBouArreridj (communes of the Daïra of El Hammadia), and their different uses in traditional medecine. A series of surveys were conducted in the study area with inhabitants and herbalists, to acquire more informations about the therapeutic uses of medicinal plants practiced by the local population. The analysis of the results allowed us to identify 78 species belonging to 36 families, the most represented are: Apiaceae (13.92%), Lamiaceae (12.65%), Asteraceae (11.39%) and Liliaceae (5.06%). The most commonly parts used from these plants are respectively: the leaves (34.21%), the stems (17.54%), the fruits (12.28%), the roots (10.52%) and the seeds (8.77%). Medicinal plants are prepared and used as powder, infusion, especially as decoction and poultice. The most used is the decoction. The main common health problem treated are; digestive disorders (29.81%), respiratory disorders (10.55%), skin inflammations (9.17%), urinary inflammations (8.25%), liver diseases (6.88%) and neurologic problems (6.42%). El Hammadia has good ethnobotanical potential of medicinal plants. This study is the first contribution to the ethnobotany of this region. We have gathered from this province some considerable knowledge about local medicinal plants for treating diseases.

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