Algerian Journal of Biosciences

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Effects Of Extraction Methods On Total Polyphenols, Free Radical Scavenging And Antibacterial Activity Of Crude Extracts Of Cleome Arabica L. Growing In Oued Souf Region
(جامعة الوادي - University of Eloued, 2020-08-31) Chouikh, Atef; Rebiai, Abdelkrim; Aref, Mahdia; Heded, Mounira; Adjal, El Hadda; Alia, Fatma
This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian). The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus.
Antimicrobial And Antioxidant Activity Of Methanol Extract Of Echinophora Spinosa L. From Jijel, Algeria
(جامعة الوادي - University of Eloued, 2020-08-31) Ghadbane, Mouloud; Bounar, Rabaha; Rebbas, Khellaf
In this study, the antioxidant and antimicrobial activities of Echinophora spinosa were investigated. Antimicrobial activity of methanol extracts obtained from Echinophora spinosa was examined using the disc diffusion method. Antioxidant activity of the methanol extracts was examined using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test. Methanol extract of ripe fruits of E. spinosa showed highest total phenolic content (69.17 ± 1.2 μg GAE/mg extract) and the major flavonoid contents (12.122 ± 0.44 μg QE/mg extract) was found in leaves of the plant. In disk diffusion antimicrobial assay, E. spinosa manifested broad spectrum of activity. The largest capacity to neutralize DPPH radicals was found for ripe fruits methanol extract of E. spinosa plant. The results shows that the various parts of E. spinosa extracts promising antioxidant and antimicrobial activities have potential bioactivities due to high content of phenolic and flavonoid compounds.
Evaluation Of The Anti-anemic Activity Of Date Syrup In Wistar Rats
(جامعة الوادي - University of Eloued, 2020-08-31) Touhami, Laiche Ammar; Ghemam Hamed, Amina; Badi, Salima
The main objective of this study is to determine the effect of food intake of date syrup on the treatment of anemia. First, we used 3 samples of dates from different regions for the preparation of the syrup. We have studied certain criteria (physical, chemical, biochemical, microbiological); where the results showed that the prepared date syrup has a good hygienic quality, and of remarkable nutritional quality due to its ideal content with many properties, such as carbohydrate and protein content. To evaluate the anti-anemic properties of date syrup in rats, anemia is induced by a food having an iron deficiency. Date syrup, prepared from Djamaa dates, was administered by gavage of anemic rats at doses of 1000 mg / kg / day and 2000 mg / kg / day resulting in an increase in hemoglobin, red blood cell count, hematocrit and serum iron. Thanks to the results obtained, date syrup can be classified among the foods that help fight iron deficiency anemia.
Boulaares, ISlam. Characterization And Acute Toxicity Evaluation Of The Mgo Nanoparticles Synthesized From Aqueous Leaf Extract Of Ocimum Basilicum L
(جامعة الوادي - University of Eloued, 2020-08-31) Derouiche, Samir; Guemari, Imane Yousra; Boulaares, ISlam
The aim of this study was to prepare magnesium oxide nanoparticles (MgONPs) using aqueous leave extract of Ocimum basilicum L. and to evaluate their acute toxicity. The characteristics of biosynthesized MgO powder was analyzed by UV–Vis spectroscopy, scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The acute toxicity test of MgONPs was applied in Wistar albino rats with different concentration. Results showed that the broad bell-shaped spectrum band was obtained by UV–Vis spectroscopy indicates the formation of MgO. The SEM images provided further insight into the shape and size of MgO which to be ranging under 440 nm. Fourier transform infrared (FTIR) spectroscopy detected the vibration of the Mg─O bond that indicate the presence of magnesium oxide nanoparticles (MgO). In this study, the toxicity test showed no mortality or behavioral change in low dose of MgNPs (250 mg / kg b.w) but we observed that 50% of rats have died when treated with high dose of MgNPs (500 mg/kg b.w.). This study confirmed that aqueous extract of Ocimum basilicum L. has potential properties as biocatalyst for the biosynthesis of MgONPs without any toxicity under dose 250 mg/kg in rats.
The Effects Of The Fertil Verde Fertilizer On The Growth And Yield Of Chili Pepper (capsicum Annuum L) In Southern Algeria
(جامعة الوادي - University of Eloued, 2020-08-31) Ghemam Amara, Djilani; Kherraz, Khaled; Alia, Zeid; Chamsa, Ahmed Khalifa; Laouedj, Hacene; Senoussi, Mohammad Mourad
A field experiment was conducted on a private farm in El Oued State of Algeria to study the efficiency of using Fertil Verde as a fertilizer by foliar spry on hot pepper seedlings. The treatment by Fertil Verde increased all growth. The increase was significant in the number of main and secondary stems, the size of the leaves, and the weight of the dry leaves. While, the weight of the wet leaves did not significantly increase. Moreover, the use of Fertil Verde has led to a significant increase in the yield qualities of the plant: the weight and shape of the fruits, fresh yield of the plant, the content of pigment, and the size of the plant. Thus, the combination of fertilizing is common and Fertil Verde is a promising low-cost option in the production of high yields.
Microbial Soft Rot Of Cultivated Fruits And Vegetables
(جامعة الوادي - University of Eloued, 2020-12-31) saliha, bourdi; Djellout, Nadine Chahrazade; Baika, Khadidja; Bamebarek, Hafsa; Benaissa, Asmaa
The nutritional richness of fruits and vegetables makes them an ideal target for microorganisms, causing in particular soft rot. The microorganisms responsible for this pathology are generally found in the environment, and are endowed with a very important enzymological power, the main action of their pathogenicity. These enzymes, which allow the degradation of the plant cell wall, allow them to cross the protective wall of the plant, causing a liquefaction of tissues, whose environmental conditions as well as transport and storage conditions favour the development of these phytopathogens. A better understanding of spoilage microorganisms and the characteristics of spoilage should allow the development of new conservation and protection technologies and reduce the loss of vegetables due to spoilage. This review summarizes the main literature data on soft rot, and the diversity and mode of action of the main microbial causal agents.
Ecodendrometric Parameters Of Some Medicinal Plants In Aleppo Pine Forests Of Northern Sétif (algeria)
(جامعة الوادي - University of Eloued, 2020-12-31) rabihat, eadaad; Bounar, Rabah; Ghadbane, Mouloud; Chermat, Sabah; Rebbas, Khellaf
The ecodendrological study, on 35 plots were sampled in the Alep pine formation of the Ain Hamda Tizi N’bechar forest at the rate of one plot per station and in each plot a Phytoecological floristic survey was carried out. Concerning the species of the floral procession of the pine forest, there are 19 medicinal species, belonging to 13 families and 20 genera. This medicinal potential must be protected and preserved; it is in this context that this study is based on the knowledge of the factors which influence the distribution and the distribution of these species within the framework of a good rational management in support of the local and sustainable development of the local population through valuation.
Integration Of Prior Information In Kaplan Meier Estimator Using Bayesian Approach
(جامعة الوادي - University of Eloued, 2020-12-31) Pempie, Pascal; Hamimes, Ahmed; Benamirouche, Rachid
As part of this contribution, we will illustrate the effectiveness of the Bayesian approach in estimating durations; we suggest a new definition of the Kaplan Meier Bayesian estimator based on a stochastic approximation under an informative prior. For this reason, based on the lognormal distribution, we have unconjugated a priori distributions. This method of processing makes it possible to assume that the use of the a priori data with the various suggested methods is sensitive to the choices of the parameters added.
Level And Health Risk Evaluation Of Heavy Metals And Microorganisms In Urban Soils Of Lagos, Southwest Nigeria
(جامعة الوادي - University of Eloued, 2020-12-31) Moustafa, Boufadina; Tajudeen, O. Yahaya; Esther, O. Oladele; Benjamin, Chibs; Abdulmalik, Abdulazeez; Kelechi, Nnochiri; Akinola, O. Stephen; Hikmat, Ahmed; Angela, Daniel
Heavy metal and microbial pollution of the environment are linked to the increasing prevalence of diseases worldwide. Accordingly, this study assessed the safety of urban soils in Lagos, Nigeria, with regard to the levels of lead (Pb), nickel (Ni), cadmium (Cd), copper (Cu), chromium (Cr), zinc (Zn), and microorganisms. Soil samples were collected from Iwaya, Makoko, and Ilaje areas of the city and subjected to atomic absorption spectroscopy (AAS) and microbiological examinations using standard protocols. The mean values obtained for the heavy metals and microorganisms were compared with the World Health Organization (WHO) permissible limits. The average daily oral ingestion (ADOI), average daily dermal ingestion (ADDI), and the hazard quotient (HQ) of the heavy metals were also calculated. The AAS indicated that the soils in the three areas contained non-permissible levels of the evaluated heavy metals, except Cu and Cd. The microbiological examinations also showed that the soils contained abnormal levels of heterotrophic bacteria (HB), hydrocarbon utilizing bacteria (HUB), heterotrophic fungi (HF), and hydrocarbon utilizing fungi (HUF). The ADOI of the heavy metals in the three areas were above the recommended levels, while ADDI were normal. Moreover, the HQ of oral ingestion of each heavy metal except Cr was greater than one, while the HQ of dermal ingestion of the heavy metals was less than one. These findings suggest that the soils could predispose the residents in the areas to diseases. Thus, there is a need for regular environmental sanitation and environmental pollution control in the areas, and personal hygiene.
Biological Control By Plant Growth Promoting Rhizobacteria
(جامعة الوادي - University of Eloued, 2020-12-31) ben arbia, radia; Bestami Merdia . Ben Malek, Rokaia; Fellan, Kheira; Benaissa, Asmaa
Plant Growth Promoter Rhizobacteria (PGPR) is soil bacteria that can live on, in or around plant tissue and promote plant growth by many mechanisms that include a biological control of plant pathogens. Indeed, PGPRs have a protective effect through several modes of action such as antagonism, competition, production of hydrolytic enzymes and biofilm formation. Moreover, the use of PGPRs as biocontrol agents is very harmonious with the environment and therefore represents a good alternative to the use of chemicals in agriculture. This review is presented as a general bibliographical synthesis on the different aspects of PGPRs and their biocontrol potential.
Synthesis And Biological Activity Of 2-((3-cyano-4,6-distyrylpyridin-2-yl) Thio) Acetamide And Its Cyclized Form
(جامعة الوادي - University of Eloued, 2020-12-31) Negmari, yousef; Shaban A. A, Abdel-raheem; Adel M., Kamal El-dean; Reda, Hassanien; Mohamed E. A., El-sayed .; Aly A., Abd-ella
In this paper, 2-((3-Cyano-4,6-distyrylpyridin-2-yl)thio)acetamide (2) and its cyclized form, 3- amino-4,6-distyrylthieno[2,3-b]pyridine-2-carboxamide (3), were prepared and their structure characterizations were performed by the means of elemental and spectroscopic analyses. Their biological activity as insecticides against cowpea aphid Aphis craccivora Koch using acetamiprid insecticide as a reference was studied. The bioassay results for compounds (2) and (3) against nymphs of cowpea aphid showed that the LC50 values were 0.192 and 0.841 ppm, respectively, after 24 h of treatment but the LC50 values were 0.041 and 0.095 ppm, respectively, after 48 h of treatment. Furthermore, the bioassay results for compounds (2) and (3) showed that the LC50 values were 1.233 and 2.949 ppm, respectively, after 24 h of treatment and the LC50 values were 0.142 and 0.270 ppm, respectively, after 48 h of treatment against adults of cowpea aphid. Given these observations, it has been found that there is a remarkable relationship between the biological activity and the structure of the used compounds.
Phytochemical Study, Nutritive Value, Antioxidant And Anti-inflammatory Activities Of Phenolic Extracts From Desert Plant Calligonum Comosum L'hér.
(جامعة الوادي - University of Eloued, 2020-12-31) Saleha, Awadi; Chouikh, Atef; Chemsa, Ahmed Elkhalifa; Aounallah, Chahira; Aounallah, Imane; Alia, Fatma
The aim of this study is to the estimation of the nutritive values, phytochemical study and the antioxidant and anti-inflammatory activities of ethyl acetate, tannins and crudes extracts from the aerial part of Calligonum comosum L’Hér. Which grow in South East of Algeria. According to the results obtained from the estimation of nutritive value, the plant is rich in proteins and lipids and very poor in carbohydrates. The results also showed a difference in the content of polyphenols and flavonoids. The crude extracts were high in polyphenols content in the ethanol extract 170.74±5.8 mg EAG/g Extract, and the best value of flavonoids in methanol extract 28.22±0.25 mg QE/g Extract. Chromatographic analysis by HPLC of methanolic extract has identified four phenolic compounds out of 38 peaks as: Gallic acid, Chlorogenic acid, Caffeic acid and Vanillic acid. The antioxidant activity was evaluated by three tests, the results of the DPPH• test, showed that methanol extract had the best scavenging than the other extracts (IC50: 2.09±0.02 μg/ml), But, in the hemolysis test, the values of the percentage of hemolysis were approximately the same at concentration 1 mg/ml between the extracts and standard used. In the reducing power test, its results showed that the tannins extract had the highest reducing power 79.17±5.22 μg/ml at Abs700nm=0.5. As for anti-inflammatory activity, the methanol and flavonoids phase ethyl acetate extracts showed the best inhibitory effect against protein denaturation compared to other extracts. The effects were estimated 3.14mg E Diclofenac/mg Extract.
Preliminary Phytochemical Screening, Quantification of phenolic compounds, of Plant Extract from Chenopodium quinoa
(جامعة الوادي - University of Eloued, 2021-06-30) KADRI, mounira; RAMDANE, Farah; BOUZID, Rachidac; ATTALI, Halima
The aim of this study was to screening the phytogenic chemical compounds of the seeds of Chenopodium quinoa, obtained from Wilaya of El-Oued. The chemical study showed that the plant contained a number of secondary metabolites, flavonoids, tannins, saponins, steroids and triterpenes, glycosids while the absence of alkaloids and coumarin in Chenopodium quinoa. Folin-Ciocalteau colorimetric were used to determine the total phenolic con-tent (TPC), in the hydroalcoholic seeds extracts. The yield of the methanolic extract was estimated at 36.66%. As for the quantitative content of polyphenols, it is 11.647 ± 1.91µg AGE / mg extrait From this, Chenopodium quinoa is considered a nutritional and therapeutic value because it contains secondary metabolites
Influence of leaf extracts and total flavonoids of Rhus tripartita (Ucria) Grande on phytobeneficial bacteria associated with its rhizosphere
(جامعة الوادي - University of Eloued, 2021-06-30) Benaissa, Asmaa; Dahbi Lalla, Fatma-Zohra; Tabra Oum, Elhassenea; Djellout, Nadine Chahrazade
The article deals with the antimicrobial effect of Rhus tripartita (Ucria) Grande leaf extracts and total flavonoids against twelve antagonists Plant Growth Promoting Rhizobacteria of its rhizosphere, characterized in a previous study. The aim of this study is to demonstrate that leaves through their decomposition in the soil, may affect the distribution of bacterial communities in the rhizosphere. Leaves extracts were performed with distilled water, alcohol, methanol, hexane and chloroform as solvent and diluted in concentrations of 0.001, 0.01 and 0.1 mg/mL. The extraction of total flavonoids was carried out from leaves’ methanolic extract. The antimicrobial effect of the extracts was evaluated by the agar diffusion method and the determination of the minimum inhibitory concentration was carried out on a liquid medium. Alcohol, chloroform and methanol extracts were found to be the most effective on tested strains. The maximum zone inhibition was 18 mm, and the minimum zone inhibition was 7 mm. Rt 1: Bacillus licheniformis appears to be the most sensitive to all extracts. In contrast, Rt 7: Bacillus megaterium, seems to be the less sensitive strain. On the other hand, total flavonoids had a significant effect on 25% of the strains tested, mainly Bacillus genus. With a broad antimicrobial spectrum, the Rhus tripartita leaf extracts can be considered as a control agent for the distribution of the bacterial community in the rhizosphere. Therefore, our work showed that the plant could influence the bacterial diversity of its rhizosphere through its leaves.
Ecology and behavioral response of Ruddy Shelduck (Tadorna ferruginea) in the wetland complex of Oued Righ valley (Algerian Sahara).
(جامعة الوادي - University of Eloued, 2021-06-30) Yassine, NOUIDJEM; Asma, ZOUBIRIa; Sakina, MEROUANIa; Fateh, MIMECHE
The wetland complex of Oued Righ valley accommodates a population of Ruddy Shelduck (Tadorna ferruginea) between 2012 and 2014, the total reached 80 individuals in the Lake Hamraia where the species wintering. The analysis of the diurnal time budget of wintering populations revealed the role of feeding area delivery for this species and they devoted their day time to feeding (51.2%), sleeping (18.8%), followed by swimming activity (6.3 %), and courtship activities with flying (2.03%) which often occurs after disturbances caused by human or by Marsh harrier (Circus aeruginosus). It is interesting to study the behavior of these ducks in Saharan bioclimatic area and follow the phenology and characteristics in order to preserve the biodiversity in this region.
Characterization of endospore-forming bacteria producing extracellular enzymes isolated from the Djurdjura Mountains in Algeria
(جامعة الوادي - University of Eloued, 2021-06-30) Merzouk, YAHIAOUI; Khelifa, BOUACEb; Katia-Louiza, ASMANI; Sondes, MECHRI; Hamdi, BENDIF; Mohammed, HARIRe; Bassem, JAOUADI
Biodiversity in mountains in Algeria appears scanty and has not been thoroughly investigated. However, the mountain soil has been shown as an almost entire reserve of novel enzymes with interesting properties for industrial and environmental applications. In the present study, thirty bacterial strains were isolated from the Djurdjura Mountains in Kabylia (Algeria) and were studied for their ability to produce enzymes to be possibly used in biotechnological processes such as amylase, caseinase, and chitinase. The characterization of these isolates was carried out using morphological, physiological, and biochemical characteristics. All the data obtained with regards to the phenotypical properties of the isolates, confirmed that the strains belonged to the Bacillus group. In addition, the 16S rRNA gene of the two retained strains KA15 and LK-DZ15 was also amplified and sequenced. Phylogenetic tree was, afterwards, constructed. The nucleotide sequences and blast analyses confirmed that the KA15 and LK-DZ15 strains were closely related to those of the Bacillus altitudinis (accession n°.: MK874318) and Paenibacillus timonensis (accession n°.: MK734103) strains. The presence of amylases, proteases, and chitinases in KA15 and LK-DZ15 isolates are an indicator of their pivotal application in a variety of biotechnological processes.
A new locality of an invasive Gecko, Cyrtopodion scabrum (Heyden,1827) in Algeria (Squamata: Gekkonidae)
(جامعة الوادي - University of Eloued, 2021-06-30) Salah Eddine, Sadine; Choyaib, Bounaba; Mayssara, El Bouhissi
The Rough Bent-toed Gecko Cyrtopodion scabrum (Heyden,1827) was recently recorded in Algeria, from the east part of countries. In this note, another new locality of this species in the central Algeria (Ghardaïa region), situated at 450 km west form the last locality, based on one specimen fond in Bord of Sebkhet. C. scabrum is listed as an invasive species and it was reported as a desert species, in our finding it was recorded in an important wetland (Sebkhet El Melah) which is classified in the Ramsar list in 2004, this statement proves once again the ecological plasticity of this species.
Ethnobotanical survey of herbal remedies traditionally used in ElHammadia (Southern region of the province of Bordj Bou Arreridj, Algeria)
(جامعة الوادي - University of Eloued, 2021-06-30) Hamdi, BENDIF; Mohammed, HARIR; Merzouk, YAHIAOUI; Nabila, SOUILAHd; Fatima Zohra, HECHAICHI; Mohamed, DjamelMIAR; Ifriqya, MEDILA
This work is devoted to the study of medicinal plants in the southern region of BordjBouArreridj (communes of the Daïra of El Hammadia), and their different uses in traditional medecine. A series of surveys were conducted in the study area with inhabitants and herbalists, to acquire more informations about the therapeutic uses of medicinal plants practiced by the local population. The analysis of the results allowed us to identify 78 species belonging to 36 families, the most represented are: Apiaceae (13.92%), Lamiaceae (12.65%), Asteraceae (11.39%) and Liliaceae (5.06%). The most commonly parts used from these plants are respectively: the leaves (34.21%), the stems (17.54%), the fruits (12.28%), the roots (10.52%) and the seeds (8.77%). Medicinal plants are prepared and used as powder, infusion, especially as decoction and poultice. The most used is the decoction. The main common health problem treated are; digestive disorders (29.81%), respiratory disorders (10.55%), skin inflammations (9.17%), urinary inflammations (8.25%), liver diseases (6.88%) and neurologic problems (6.42%). El Hammadia has good ethnobotanical potential of medicinal plants. This study is the first contribution to the ethnobotany of this region. We have gathered from this province some considerable knowledge about local medicinal plants for treating diseases.
Etude de la stabilité de quelques variétés de blé dur (Triticum durum Desf.) dans les conditions semis aride du Sétif en Algerie.
(جامعة الوادي - University of Eloued, 2021-06-30) GUENDOUZ, Ali; OULMI, Abdelmalek; FRIH, Benalia; HAZZEM, Abdelhalim; MECHROUK, Walid
Notre objectif est d’etudier la stabilité du rendement de dix genotypes de blé dur expérimentées pendant quatre ans (2009-2013) dans la région semi-aride de Sétif.Nous utilisons au cours de cette étude trois indices de stabilité paramétriques et non paramétriques pour évaluer les performances des génotypes. L’étude des indices paramétrique de stabilité, notamment l'écovalence de Wricke (Wi²) a montré que les génotypes Mexicali75 (4.23) et Hoggar (11.07) sont considérés comme les plus stables parmi les génotypes testés. Alors qu'en termes d'indice de supériorité (Pi), les genotypes Polonicum (29,61), Sooty (33,99), Altar84 (34,47) et Mexicali75 (35,37) ont les valeurs les plus faibles de (Pi). L'indice d'adaptabilité géométrique (GAI) a montré que les génotypes Mexicali75 (42,00), Kucuk (40,26) et Altar84 (40,03) sont les plus stables. Les indices non paramétriques Si(2), Si(3), Si(6) ont des corrélations non signifiantes avec le rendement moyen en grains. L’étude de la variabilité génotypique du rendement en grains a montré que Mexicali75 était le génotype qui avait la plus petite variation de rendement au cours des quatre saisons. Globalement, Mexicali75, Hoggar et Kucuk sont les génotypes très stables.
In-vitro Antioxidant activity, Biosafety, Nociceptive and Antiinflammatory potential of Acetone Polyherbal (ELNA) Extract in mice
(جامعة الوادي - University of Eloued, 2021-12-30) MacDonald, Idu; Benjamin, Ogunma Gabriel; Chike Uzochukwu, Onuigbo
ELNA (Moringa oleifera, Crateva religiosa and Curcuma longa) is a polyherbal formulation comprising of Moringa oleifera, Crateva religiosa and Curcuma longa. It is used traditionally in the treatment of inflammation, gastro-intestinal infections, hypertension and immune compromised diseases. This study investigate the in-vitro anti-oxidant capacity, biosafety, nociceptive and anti-inflammatory potential of polyherbal ELNA acetone extract. In-vitro antioxidant study was done on the extract using a standard protocol of 1, 1, Diphenyl 2, picrylhydraxyl (DPPH) radical scavenging activity. Toxicological profiling was done using standard methods. Acetic acid, hot plate and egg albumin models were designed for the nociceptive and anti-inflammatory properties of the extract. DPPH scavenging property of the extract showed significant increase at graded concentration. Acute toxicity study of ELNA acetone extract revealed no toxic effect, with absent mortality and less adverse effect (LD50>5000 mg/kg body weight). The sub-chronic toxicity study of the acetone extract for 28 days, showed no significant difference (p= 0.05) in the organ to body weight ratio. The haematological indexes indicated no significant different except at 400 mg/kg that elicited slight significant (p<0.05) increase in the platelet. The results showed that graded doses of the extract at (400, 800 and 1200 mg/kg body weight) exhibited significant (p<0.05) decrease in peripheral and central pain also a decrease in the paw edema volume of inflammation. This was achieved in dose dependent manner. The result of this study established a pharmacological evidence for the traditional use of ELNA as an analgesic and anti-inflammatory agent, it also present information on the anti-oxidant properties and toxicity profile of the formulation.

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