International Journal of Biological and Agricultural Research

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Influence of culture media on the production of antibacterial compounds by Streptomyces sp. PAL114 isolated from palm grove soil in Ghardaïa
(University of Eloued جامعة الوادي, 2018-06-01) Tata, Samira; Yekkour, Amine; Chaouch, Fawzia Chaabane; Bouras, Noureddine; Sabaou, Nasserdine
A Saharan actinobacterium, PAL114, isolated from Ghardaïa (Algeria), showed an important antibacterial activity against various pathogenic bacteria tested on different culture media. The results showed that the highest antibacterial activity was obtained after 7 days of growth. The Bennett medium was found to be the best medium for optimal growth and antibiotic production. The secondary metabolites were detected by reverse-phase HPLC using a C18 column and the fractions corresponding to peaks were collected, concentrated then tested against Listeria monocytogenes ATCC 13932 to detect the active fractions.
Epidermal anatomic characterization of spontaneous plants belonging to Poaceae, Cistaceae and Boraginaceae families in Algerian northern sahara
(University of Eloued جامعة الوادي, 2018-06-01) SLIMANI, Noureddine; MAHBOUB, Nasma; CHEHMA, Abdelmadjid; HUGUENIN, Johann; BARIR, Asma; BEZZA, Imane Fatma
Microscopic examination of the epidermis of wild plants contained in the feces of the dromedary and ruminants is one of the methods for studying the animal diet. This work is a step to elaborate a reference catalog of epidermis to determinate the diet of camel and ruminants in the Saharan rang land situated in Algerian northern Sahara. In this paper, we described the most important epidermal anatomical characteristics of spontaneous perennial plants and ephemeral (08 species and 03families) grazed on Saharan rang land, by using an binocular light microscope. From the results, we propose an identification key to these 08 species from their epidermal characters.
Investigation of the Oxidative Stress markers in salivary, serum and erythrocyte ofEl-Oued (Algeria) Diabetic Patients
(University of Eloued جامعة الوادي, 2018-06-01) DEROUICHE, Samir; HABITA, Asma; DEGACHI, Ouidad; NECIB, Maroua; CHETEHOUNA, Roumaissa
The aim of this study was to evaluate the state of oxidative stress during diabetes in El-Oued (Algeria) patients. The experimentation is carried out on 72 voluntary individuals were divided into three groups. The first group of healthy individuals (controls), the second group diabetic type 1and the third group diabetic type 2, on which we measured some biochemical parameters on serum, erythrocytes and saliva. The Results showed a significant decrease in serum uric acid concentration in diabetics type 1 (10.78%) and diabetics type 2 (26.83%) and a significant decrease (p <0.05)in erythrocytes GSH in both types of diabetes compared to controls. On the other hand, there was a significant increase (p <0.05) in serum and salivary peroxidation lipidique (MDA) in type 1 and 2 diabetics compared to non-diabetics. In addition, a significant correlation (p <0.05) between serum and salivary MDA in type 1 diabetics (R2 = 0.750) and type 2 diabetics (R2 = 0.768) was shown in this study. In conclusion the present study reveals that diabetes induces an imbalance of oxidant / antioxidant status at serum, erythrocyte and salivary level and that salivary MDA is a good indicator of the state of oxidative stress in diabetes.
Floristic diversity of Saharan wetlands in the region of Oued Righ, northeastern Sahara of Algeria
(University of Eloued جامعة الوادي, 2018-06-01) HALIS, Youcef; BENHADDYA, Mohammed Lamine; Khellou, Mayada; Hadjoudj, Moussa; Benhania, Amel
The main objective of this study was to investigate the floristic composition and the vegetation structure of different Saharan wetlands in the region of Oued Righ. Six (6) Saharan wetlands were examined; the Lake of Merjaja, Lake of Megarine, Lake of Sidi Slimane, lake of Ayata, Lake Ain Zerga, and Oued Khrouf. A total of 25 stands were investigated using the quadrat method, with five stands for each wetland. The different vegetation parameters, such as cover, frequency and density were calculated. A total of 24 plant species belonging to 20 genera and 13 families were identified. Chenopodiaceae was the predominant family with 6 species. The majority of the species were of Saharo-Arabian distribution. Chemaephytes had the highest contribution to the life forms spectra. The potential role of the halophyte species is discussed. These results might contribute to a better understanding of the functions, requirements, and sensitivities of these ecosystems.
Therapeutic aptitude of fermented camel milk: case of antimicrobial activity of whey proteins
(University of Eloued جامعة الوادي, 2018-06-01) MOSBAH, Said; BOUDJENAH-HAROUN, Saliha; EDDOUD, Amar; BOUAL, Zakaria
The objective Whey proteins (WP) are highly functional elements containing bioactive peptides. The aim of this study is to evaluate the effect of spontaneous fermentation on the antimicrobial activity of WP in camel milk. Samples of the milk are analyzed in the raw state and after the fermentation. The pH and acidity are determined as well as the enumeration of lactic acid bacteria (LAB). The antimicrobial activity was carried out by the agar well diffusion assay, adapting the good method. The results shows that the fermentation process has shown that the pH value is decreasing and LAB show a significant increase during fermentation. The antimicrobial activity gave a very significant difference between fermented camel whey proteins (FCWP) and raw camel whey proteins (RCWP). Then the FCWP gives a zone inhibition (ZI) of 17, 16.5, 13.25 and 10.5 mm respectively for S. aureus, P. aeruginosa, K. pneumoniae and E. coli. In addition, the RCWP gives a ZI of 16, 9.5 mm respectively for S. aureus and P. aeruginosa, and 0 mm for K. pneumoniae and E. coli. These results indicate that the fermentation process induced by the development of LAB favors the proteolysis of WP, which increases the antimicrobial activity.
Influence of salt stress on the mineral composition of Arthrospira platensis grown in an extreme environment in southern Algeria
(University of Eloued جامعة الوادي, 2018-12-01) LAICHE, Ammar Touhami; BESSEI, Abdelmalek
The salinity of Saharan waters poses a problem for the growth of aquatic species. The cultivation of spirulina requires a culture medium with a salinity of about 13 g / l. The study consisted in the cultivation of spirulina in environments that differ in their salt content; D0: a control, D1: 13g / l, D2: 26g / l and D3: 39g / l. Daily reports of pH and electrical conductivity have been made. After obtaining the biomass, the determination of the level of certain spirulina mineral elements (Ca ++, Na + , K + , Mg ++, Fe ++) was carried out. According to the results, salinity does not seem to be a limiting factor for the growth of this species. Moreover, it seems that the salinity factor has little influence on the mineral composition of Spirulina, with a variability of pH values and electrical conductivity. Therefore, the cultivation of this species of seaweed in Saharan waters affected by a high salt content, seems possible and possible
Investigation of renal toxicity induced by neonicotinoid (thiamethoxam) insecticide in mice
(University of Eloued جامعة الوادي, 2018-12-01) Saadi, Leila; Raki, Ahmed Aziz; Bouazza, Marwa
The investigation of the toxicity of a neonicotinoid insecticide, thiamethoxam, in mammals has been the subject of several experimental studies. In the present work, we are particularly interested in studying the renal toxic effects of two doses (0.2 and 0.4 mg/kg/day) of thiamethoxam, an insecticide widely marketed in Algeria, administered orally in adult male mice. For this purpose, a renal histopathological study and plasma assays of the uremia and the creatinemia are carried out. Examination of the histological sections recorded dose-dependent lesions in the renal parenchyma. Renal impairment is characterized by the destruction of certain glomeruli and the presence of some hemorrhagic and inflammatory foci. These alterations are associated with leukocyte diapedesis presented in the blood vessels. The renal function is maintained in the normal state because the urea and creatinine contents remain unchanged. On the basis of these results, it turns out that thiamethoxam treatment induces a dose-dependent renal toxicity in male mice.
Evaluation of total phenolic contents and antioxidant potentials of ten medicinal plants from Algerian Sahara
(University of Eloued جامعة الوادي, 2018-12-01) DJAHRA, Ali Boutlelis; BENKADDOUR, Mounia; ZEGHIB, khaoula; BENKHERARA, Salah; OUALABI, Katrenada; GHANIA, Ahmed; JDIDI, Khaoula
In the context of discovery a new antioxidants from natural sources. We are interested in this work to study the phenolic compounds and the antioxidant activities of aerial part extracts for ten (10) medicinal plants, from Wilaya of El-Oued (South-east Algeria) : Bassia muricata, Traganum nudatum, Haloxylon scoparium, Cornulaca monacantha, Malcolmia aegyptiaca, Retama raetam, Heliathemum lipii, Zygophyllum album, Ephedra alata, Moltkia ciliate . The results obtained demonstrated that phytochemical screening of aerial part extracts for ten plants studied varies from one plant species to another. The richness of Hiliantimum lippii and Retama raetam in polyphenols whose content varies between 134.67 and 133.33 mg AGE/g Ext respectively. For other plants, the polyphenol content is between 31.02 and 58.33 mg AGE/g Ext. The antioxidant activity tests by using the DPPH method show that all the extracts of the plants studied have antioxidant properties lower than ascorbic acid (IC50 = 16.25 μg / ml) of which the species Hiliantimum lippii represents the best capacity (IC50=27.79μg/ml). The low power is recorded in the species Haloxylon scoparium (IC50 = 793.29 μg / ml)
Evaluation of the effect of spinosad on lipid peroxidation and antioxidant markers in female mice
(University of Eloued جامعة الوادي, 2018-12-01) Saadi, Leila1; Bouazza, Marwa; Mokrane, Ahlam; Aicha, Meriem; Lebaili, Nemcha
A Like any synthetic substance, or even natural, pesticides can produce harmful effects on human and animal health. The purpose of the present study is to evaluate the oxidative profile following the exposure of female mice to 9.2 mg/kg/day of spinosad. This is to examine the relationship between insecticide exposure and the risk of developing oxidative stress. Adult mice of the Naval Medical Research Institute were given by gavage daily for 18 days with spinosad, at rate of 9.2 mg/kg/day. Short-term exposure caused lipid auto-oxidation, expressed as an increase in malondialdehyde concentration in treated mice. Lipid peroxidation is associated with high level of plasma total anti-oxidative power and low level of plasma vitamin C. These results support the toxicity of the bioinsecticide witch induce oxidative stress in female mice
New data on Psammodromus blanci (Lataste, 1880) a near threatened species of Lacertidae (Reptilia: Squamata)
(جامعة الوادي - University of Eloued, 2019-06-16) EL BOUHISSI, Mayssara; DAHMANI, Walid; SADINE, Salah eddine
The Blanc's sand racer Psammodromus blanci (Lataste, 1880) is a small species of Lacertidae (Squamata). This species occurs the North West of Algeria and it is noted as very rare in Morocco and Tunisia. According to the International Union for Conservation of Nature (IUCN) assessment of 2009, this species is listed as near threatened. The objective of our work is to update the information about this species basing on recent investigations between May and June 2019 in Sidi Bel-Abbes (North West of Algeria). Some remarks on the activity and ecology of this species are also noted with describing of a new locality in Aflou (Laghouat) North West of Algeria.
Biofungicide Activity of Datura stramonium Leaf Extract Against Phytopathogenic Fungi
(University of Eloued جامعة الوادي, 2019-06-16) DJAHRA, Ali Boutlelis; BENKADDOUR, Mounia; ZEGHIB, Khaoula; BENKHERARA, Salah; SHAIEB, Inas; GHANIA, Ahmed; BDIDA, Soumaia
The aim of this study is determination of polyphenol contents and biofungicide activity of crude extract of Datura stramonium. The results of extraction showed an important yield percentage of crud extract equal 25.22 % wherase, the polyphenol content of the extract was 657,0 (mg AGE/ml Ext). The In vitro biofungicide activity carried out by disk diffusion method, the biofungicide effect results performed with crude extract and Milraz WP 76 fungicide (Propineb 70%, Cymoxanil 6%) against fungal strains. The results appear that natural substances possess an antifungal power on the multiresistant germs responsible for infectious diseases for plants. Growth inhibition varies depending on the fungal strains and the concentration of the test product
Extraction and characterization of the antioxidant activity of essential oils from cotula cinerea in the region of el oued (algeria)
(جامعة الوادي - University of Eloued, 2019-06-16) MAHBOUB, Nasma; SLIMANI, Noureddine; HENNI, Meriem
This work is part of the valorization of spontaneous medicinal plants, a large part of which is still virgin and requires in-depth studies, and then our objective will be an extraction of the essential oils of the aerial part and the flowers of Cotula cineria, identifying physical parameters, pH measurement, density measurement, and refractive index. In parallel, biological activity (Antioxidant) is studied. Regarding the physical properties, we find the essential oils of the flowers and the aerial part have basic characters with the values of pH 8,39 and 8,60, the density is 0.95 and 0.90 and the index of refraction is 1,33350 and 1,33375 respectively. Both antioxidant activities show that both essential oils have IC50 45.98mg/ml and 79.28mg/ml for the flowers and the aerial part of Cotula cinerea respectively. A large ability to trap the DPPH radical is noted with the value of IC50 0.04% for ascorbic acid.
Ameliorative effect of Aristolochia longa and Aquilariamalaccensison lead acetate inducednephrotoxicityin female rats
(University of Eloued جامعة الوادي) DEROUICHE, Samir; GHARBI, Safa; ZEGHIB, Khaoula; KHELEF, Yahia
Background:Aquilariamalaccensis And Aristolochia longa are plants used extensively in traditional medicine for the treatment of cancer in several regions in Algeria. We investigate the ameliorative effect of Aristolochia longa and Aquilariamalaccensison kidney toxicity induced by lead in female albino rats. Methods:Twenty five (25) apparently healthy female Wistar rats were randomly divided into five groups of five rats in each: control, Pb, Pb + A. longa (Ar), Pb+ A. malaccensis (Aq), and Pb+Ar+Aq. lead (100 mg/kg b.w) as Pb(C2H3O2)2 added in their drinking water for 75 days. A. longa (rhizome powder at a dose 1% w/w of diet) and A. malaccensis (heartwood powder at a dose 1% w/w of diet) were added to the feed during the last 15 days of lead exposed in the animals. Results:Obtained results revealed that lead treatment caused a significant increase in blood glucose level, serum urea, creatinine and uric acidlevel and in kidneyof MDA level and CAT activity. In contrast, it led to an decrease in the kidneyGST activityand GSH level in rats .Also, the results clearly showed that lead causes alterations of glomerular and tubular structure in comparison with controls.Our results showed that treatment with A. malaccensis and A. longa a partial correction of the previous parameters.The histological observations confirmed the nephroprotection results by the biochemical parameters. Conclusion:Results demonstrated beneficial effects of A. longa and A. malaccensis treatment againacetate lead-induced toxicity and kidney damage in rats

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