Influence of culture media on the production of antibacterial compounds by Streptomyces sp. PAL114 isolated from palm grove soil in Ghardaïa

Abstract

A Saharan actinobacterium, PAL114, isolated from Ghardaïa (Algeria), showed an important antibacterial activity against various pathogenic bacteria tested on different culture media. The results showed that the highest antibacterial activity was obtained after 7 days of growth. The Bennett medium was found to be the best medium for optimal growth and antibiotic production. The secondary metabolites were detected by reverse-phase HPLC using a C18 column and the fractions corresponding to peaks were collected, concentrated then tested against Listeria monocytogenes ATCC 13932 to detect the active fractions.