Algerian Journal of Biosciences

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Browsing Algerian Journal of Biosciences by Author "Alia, Fatma"

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    Effects Of Extraction Methods On Total Polyphenols, Free Radical Scavenging And Antibacterial Activity Of Crude Extracts Of Cleome Arabica L. Growing In Oued Souf Region
    (جامعة الوادي - University of Eloued, 2020-08-31) Chouikh, Atef; Rebiai, Abdelkrim; Aref, Mahdia; Heded, Mounira; Adjal, El Hadda; Alia, Fatma
    This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian). The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus.
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    Phytochemical Study, Nutritive Value, Antioxidant And Anti-inflammatory Activities Of Phenolic Extracts From Desert Plant Calligonum Comosum L'hér.
    (جامعة الوادي - University of Eloued, 2020-12-31) Saleha, Awadi; Chouikh, Atef; Chemsa, Ahmed Elkhalifa; Aounallah, Chahira; Aounallah, Imane; Alia, Fatma
    The aim of this study is to the estimation of the nutritive values, phytochemical study and the antioxidant and anti-inflammatory activities of ethyl acetate, tannins and crudes extracts from the aerial part of Calligonum comosum L’Hér. Which grow in South East of Algeria. According to the results obtained from the estimation of nutritive value, the plant is rich in proteins and lipids and very poor in carbohydrates. The results also showed a difference in the content of polyphenols and flavonoids. The crude extracts were high in polyphenols content in the ethanol extract 170.74±5.8 mg EAG/g Extract, and the best value of flavonoids in methanol extract 28.22±0.25 mg QE/g Extract. Chromatographic analysis by HPLC of methanolic extract has identified four phenolic compounds out of 38 peaks as: Gallic acid, Chlorogenic acid, Caffeic acid and Vanillic acid. The antioxidant activity was evaluated by three tests, the results of the DPPH• test, showed that methanol extract had the best scavenging than the other extracts (IC50: 2.09±0.02 μg/ml), But, in the hemolysis test, the values of the percentage of hemolysis were approximately the same at concentration 1 mg/ml between the extracts and standard used. In the reducing power test, its results showed that the tannins extract had the highest reducing power 79.17±5.22 μg/ml at Abs700nm=0.5. As for anti-inflammatory activity, the methanol and flavonoids phase ethyl acetate extracts showed the best inhibitory effect against protein denaturation compared to other extracts. The effects were estimated 3.14mg E Diclofenac/mg Extract.