EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE EFFECT

Abstract

Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically Marine sponges are oldest metazoans which were found to have structural and biologically diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge diverse lectins. A lectin isolated from marine sponge Axinella donnani Axinella donnani Axinella donnani (ADL) were (ADL) were (ADL) were (ADL) were (ADL) were (ADL) were evaluated evaluated evaluated evaluated evaluated for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cyt Guanidine hydrochloride does not show any activity. Finally, cytGuanidine hydrochloride does not show any activity. Finally, cyt otoxicity of otoxicity of otoxicity of otoxicity of otoxicity of ADL at different at different at different at different at different at different at different at different concentrationsconcentrations concentrationsconcentrations concentrationsconcentrations concentrations (0.25 -500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF500 mg/mL) was tested against MCF 500 mg/mL) was tested against MCF-7, A431 and HeLa 7, A431 and HeLa 7, A431 and HeLa 7, A431 and HeLa 7, A431 and HeLa 7, A431 and HeLa 7, A431 and HeLa cellell lines by ines by MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these MTT assay. ADL exhibited potential cytotoxicity against these cel l lines after 24 hours in a lines after 24 hours in a lines after 24 hours in a lines after 24 hours in a lines after 24 hours in a lines after 24 hours in a lines after 24 hours in a lines after 24 hours in a lines after 24 hours in a