Phytosynthesis of CuNPs by Medicago sativa and their effects on some biological and oxidative stress parameters in experimental Anemia induced by phenylhedrazine in rats

Abstract

This investigation was aimed to study the effects of the aqueous extract of Medicgo sativa and copper nanoparticles CuNPs on certain biochemical, hematological and oxidative stress parameters in phenylhydrazine induced experimental anemia in female wistar rats. The experimental study carried out in the laboratory on 20 female Wistar rats were divided into four groups, the first group rats serves as a control, the second is experimental anemia rats (PHZ), and the third is experimental anemia rats treated by copper nanoparticles (3 mg/kg) (PHZ+CuNPs) and the fourth is experimental anemia rats treated with the aqueous extract of M. sativa (200 mg/kg) (PHZ+MS). Some biochemical, hematological and oxidative stress markers were determined. Biosynthesized CuNPs were characterized by analytical methods. Results of in vitro phytochemical and HPLC analysis results revealed that M. sativa contains most of active compounds, especially phenolic (naringin and vanilic acid) with high antioxidant activity. Characterization of CuNPs confirmed the involvement of biological molecules in CuNPs synthesis with size ranged from 19.8 to 92.8 nm. Results of in-vivo rats study showed a change in hematological and some biochemical parameters in experimental anemia group compared to control. Also, results illustrated an oxidative stress in PHZ rats with high level of MDA and low level of SOD compared to control. Histopathology of bone, spleen and kidneys tissues tested confirmed the altered in these tissues with inflammation, necrosis, hemorrhagic in anemia rats compared to control. However, the obtained Results show a partial improvement of all of previous alteration in rats treated with M. sativa plant and CuNPs compared to infected rats. In conclusion, the present study suggests that CuNPs and M. sativa extract could be a substantially promising anti-inflammatory and antioxidant effect which can be used in treatment of anemia.