EVALUATE OF THE ANTIOXIDANT ACTIVITY OF POTATOES BY RADICAL SCAVENGING ACTIVITY USING O•2− AND DPPH

Abstract

This work aimed to evaluate antioxidant capacity of four kinds of Solanum tuberosum L (potato), the total phenolic content of ethanolic extracts of whole roots shows values ranged from 19.21 to 08.88 mg AG/g of extract, the amount of total flavonoids content varied from 25.03 to 10.91mg rutin/g of extract. However, this phytochemical content of sample extracts had an effect on the capacity antioxidant which measured with scavenging radical activity, was carried out spectroscopy assay by using DPPH radical and cyclic voltammetry assay for superoxide anion radical (O•2−). The IC50 values ranged from 1.21 to 3.86 mg/ml and from 1.03 to 1.81 mg/ml for the inhibition of DPPH and superoxide anion radical respectively. Also, the diminution of the current peak of (O•2−) in absence and presence of sample extract was successfully investigated for the determination of interactions parameters as binding constant Kb, the ratio of binding constants, binding free energy and mode of interaction. For these results, there was a significant statistically (p<0.005). All the Solanum tuberosum L showed appreciable levels of antioxidant activities and can be promoted as a natural antioxidant preference in food.