Phytochemical study, biological evaluation and Ag Silver nanoparticle synthesis using Psidium guajava leaf extracts.

Abstract

Our study was based on a biological examination of extracts of the leaves of Psidium guava, a member of the Myceliaceae family, harvested from the Guemar region of Oued Souf province. The study sample was prepared, and the methanolic extract, essential oil, and silver nanoparticles (AgNPs) were obtained. The amount of polyphenols was estimated at (17.889 ± 0.167 mg gallic acid equivalent/g) and flavonoids at (0.313 ± 0.001 mg quercetin equivalent). Based on the results of evaluating the antioxidant activity of the extracts using the DPPH and FRAP assays, the results showed that the IC50 value for the methanolic extract was estimated at (0.984 ± 0.023 mg/ml), the IC50 value for the essential oil was estimated at (3.571 ± 0.179 mg/ml), and the IC50 value for the nanoparticles was estimated at (18.891 ± 1.4 mg/ml). We then conducted an experimental study on a rat model to test the extracts' ability to treat burns. Polyphenols, alkaloids, and essential oil were extracted from Psidium guava leaves using appropriate extraction techniques, while

silver nanoparticles (AgNPs) were prepared from the polyphenol extract. A 1cm diameter burn was inflicted on the dorsal surface of mice. The extracts were then applied at different concentrations topically to the burn in the rats model, compared with a positive control group (Mebo ointment was used) and a negative control group (burns were inflicted and left untreated). The efficacy of the extracts was evaluated over 12 days by measuring the burn shrinkage rate. The results showed that all Psidium guava leaf extracts (polyphenols, alkaloids, essential oil, and

silver nanoparticles (AgNPs)) exhibited significant activity in promoting burn healing.