In-vitro and in-vivo antifungal effect of silver oxide and Syzygium aromaticum in Wistars rats

Abstract

The objective of this work is to study the therapeutic effect of the aqueous extract of S. aromaticum and silver nanoparticles AgNPs on certain biochemical, hematological and oxidative stress parameters and on liver and testis histological in male wistar rats infected by Aspergillus niger. The experimental study carried out in the laboratory on 25 male Wistar rats weighing 130-245 g were divided into four groups, the first group serves as a control, the second is contaminated by Aspergillus niger spores, and the third is contaminated by fungal spores and treated with AgNPs nanoparticles (80 µg/kg) and the fourth is contaminated with fungal spores and treated with the aqueous extract (200 mg/kg). Some biochemical, hematological and oxidative stress markers were determined. Biosynthesized AgNPs were characterized by analytical methods and the in-vitro antifungal activity was applicated using standard methods. Results of in vitro phytochemical and HPLC analysis results revealed that S. aromaticum contains most of active compounds, especially flavonoids (naringin and chlorogenic acid) with high antioxidant activity and without toxicity in rats. But the AgNPs has a little toxicity in rate up a dose (800µg/ kg). Characterization of AgNPs confirmed the involvement of biological molecules in AgNPs synthesis with size ranged from 19.8 to 92.8 nm and the average diameter was 46 nm. Results of in-vivo rats study showed a change in hematological and some biochemical parameters in rats infected by fungal spores compared to control. Also, results illustrated an oxidative stress in rats infected by Aspergillus niger with high level of MDA and low level of GSH compared to control. Histopathology of liver and testis tissues tested confirmed the altered in these tissues with inflammation, necrosis, hemorrhagic in rats infected by fungal sport compared to control. However, the obtained Results show a partial improvement of all of previous alteration in rats treated with S. aromaticumplant and AgNPs compared to infected rats. In conclusion, the present study suggests that AgNPs and S. aromaticum extract could be a substantially promising anti-inflammatory, antioxidant and antifungal effect which can be used in treatment of disease caused by this fungal. Aspergillus niger, Syzygium aromaticum, AgNPs, Caracterization, Oxidative stress, inflammation, Wistar rats.