Evaluation of anti inflammatory activity Chamaeleo Chamaeleon's skin against nickel toxicity in wistar rats

Abstract

Saharan culture is very rich in theory which stops us to research on the use of chamaeleon dry skin. This investigation was aimed to study the effect of ethanolic extract of the C.Chamaeleondry skin, Twenty male albino Wistar rats, weighed 180±15 g were divided into 4 groups of 5 animals each (n = 5);Group 1: normal control, Group 2:rats exposed to nickel (20mg / kg) and received normal saline, Group 3: rats exposed to nickel (20mg / kg)was treated with ethanolic extract of C.Chamaeleon dry skin (300mg/kg), Group 4: rats exposed to nickel (20mg / kg)was treated with ethanolic extract of C.Chamaeleon dry skin (400mg/kg) for 7 days. Various parameters as hematological, biochemical markers were estimated, In this study, the toxicity test showedan increase in relative liver weight, The results also showed notable changes inbiochemical parameters characterized by a significant increase in theserum concentration of glucose, urea and creatinine serum.However, the activities of transaminases (TGO, TGP) and C-Reactive Proteinserum have shown an increasein rats contaminated with nickel in compared to control. Treatment with C.Chamaeleon almost normalizes the parameters biochemical and hematological parameters. After the extraction processes, the yield of the extraction was (20.78%).Results of physicochemical and biochemical analysis revealed that the dry skin contains respectively 92.74% of dry matter, 77% of ash, 0.99% of protein, 8.3% of carbohydrates and 2.73% of lipid. The results of antioxidant activity showed that theIC50 values of the skin dry was 46.02 mg/ml.FRAP value 4.43±0.09 (mg equivalent of ascorbic acid /g of extract). The ethanolic extract did not show any activity against the bacterial strains. However the results of haemolysis test showed highest haemolytic effect (84.65%) at the concentration of 1000μg/ml. The study reveals anti- inflammatoryproperty was observed in ethanolic extract of C.Chamaeleon dry skin in groups treated with nickel.