.دراسـة الفـاعلية البيولـوجية والفيتوكيميائية لنبـات تـوقفت النامي بمنطقة تبسة

Abstract

The objective of this study was to conduct a qualitative test of the biological efficacy of the plant Artemisia campestris L. A sample of the plant was taken in the wilaya of Tebessa (eastern Algeria). After drying and grinding, a series of tests were carried out. The quantitative content of the organic matter was estimated based on the protocol reported by Shibko et al. (1966), in which we recorded a significant variation in the amount of primary metabolites, where the carbohydrate compounds had the highest value of 157.07 mg E glucose/g, followed by the protein compounds which recorded 98.14 mg E/BAS/g Ms, while the lipid compounds had the lowest value of 14.951 mg E/Soja/g Ms. From the phytochemical perspective, the maceration method was used to obtain the crude aqueous extract, the yield of which was estimated at 15%. We also quantified the total phenolic content, and the results showed that the sample contained a considerable amount of phenols, estimated at 47.531 mg E AG/g Ex. Additionally, the flavonoid content was quantified and estimated at 9.591 Mg E Qu/g Ex. The antioxidant activity was also tested using the DPPH• free radical, where the value was estimated at 0.135 mg/ml in the crude extract of Artemisia campestris L. The ferric reducing antioxidant power (FRAP) was estimated at 0.399 mg/ml, and the total antioxidant capacity (CAT) was recorded at 11.44 mg/ml. The results obtained showed that the plant has high efficiency in the DPPH• free radical test and the CAT test, and weak in the FRAP test, compared to the reference compounds. As for the anti-inflammatory activity, the plant extract showed a significant effect, estimated at 26.161 mg/ml, while it showed a very weak result in the sun protection factor (SPF) test, which was recorded at 3.52.