دراسة مقارنة للفعالية البيولوجية و الكيميائية للمستخلصات العادية و النانومترية لنبات السعد

Abstract

In the comparative evaluation of crude extract and nano-formulated extracts of tubers plants Cyperus rotundus, it was found that the yield of the crude extract was 13%, while that of the nano-formulated extract was 4%. FTIR analysis revealed the presence of various functional groups in the crude extract, with a prominent peak at 1000 cm⁻¹. Additionally, several characteristic groups were recorded in the 3000–3500 cm⁻¹ range. Regarding antioxidant activity assessed using the DPPH• free radical scavenging assay, the crude extract exhibited of nano-formulated extract, indicating a stronger antioxidant potential for the crude extract. Similarly, its reducing power was superior, with an EC₅₀ compared to for the nano-formulated extract. In the antimicrobial activity assay, both extracts showed varying degrees of effectiveness, with the IC₅₀ of the crude extract is higher whereas that of the nano-formulated extract is less. The crude extract demonstrated greater inhibition of the growth of tested microorganisms, especially pathogenic bacteria, with inhibition for Ecoli, for Streptococcus p. In conclusion, the crude extract overall demonstrated more potent antioxidant and antimicrobial activity than the nano-formulated extract, particularly against Escherichia coli and Streptococcus pneumoniae.