Preparation and characterization of some polyherbal formulations by using plants and evaluation of their biological activities in Oued souf / Righ

Abstract

Polyherb is a selective mixture consisting of two or more herbs in order to treat a specific disease or many diseases. The aim of this work was to do a statistical study on plants used in several treatments such as cancer in the El Oued region and preparation of some polyherbal formulations by using some local plants and evaluation of their biological activities. For statistical study, 61 persons interrogated were chosen, interviews were carried according to a profile of the interviewed people, cancer- related characteristics and plants and their uses. For in-vitro study, new three polyherbal (F1, F2 and F3) formulated from the most popular plant in the El-Oued area. In –In-vitro analysis, Qualitative and quantitative phytochemical compounds for plants and herbal formulations were assessed by using standard protocol. Antioxidant activity was determined by DPPH and FRAP assays, Anti- inflammatory activity were assayed by hemolysis and protein denaturation tests and antidiabetic activity were tested on different herbal formulations. Results of statistical study show that Ephedra alata, Curcuma long L., Nigella sativa L., Portulaca oleracea L. and Atriplex halimus L. which are the most popular plant used in cancer treatment in the El-Oued region. Results of the phenols and flavonoids contents assay, plants and formulations were richness of the phytochemical components, as well as significant quantities of phenols, with preference for F2 by more than 102.29 mg/g. Also, through the results of study, we obtained that the formulations have an important antioxidant activities with a slight preference for formulation F1 (IC50=24.14mg/ml for FRAP test). In the other hand, As well as with regard to anti- inflammatory activity, our results confirm that the herbal formulations have significant activity, with F3 has maximum hemolysis activity (IC50=0.108 mg/ml) and the F2 has the maximum activity of Protein denaturation (IC50=0.059mg/ml). As for anti-diabetic activity, the results confirm that the herbal formulations have anti-diabetic activity, with preference for formulation F3 which was the best one. In conclusion, through this study, we conclude that herbal formulations produced from plants has a greater biological effectiveness than plants with great activity from F2 and F3, which encourages its use in diseases related to cancer, infections, and diabetes.