Improving natural formulations to combat the Tribellium castaneum insect
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Date
2025
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جامعة الوادي university of eloued
Abstract
This research explored the potential of enhancing essential oil formulations as ecofriendly alternatives to conventional pesticides, addressing the growing need for sustainable 
pest management. The study investigated the insecticidal properties of essential oils, 
particularly peppermint oil, in addition to environmentally friendly synthetic compounds.
The methodology involved conducting toxicity bioassays on Tribolium castaneum larvae 
to assess the effectiveness of peppermint oil formulations and the synthetic compounds with 
adjuvants including xanthan gum, citric acid, and glycerol,. Phytotoxicity tests were also 
performed on lettuce leaves to evaluate the safety of these formulations for plants.
Key findings revealed that peppermint oil (NF1) demonstrated significant insecticidal 
activity with a 76% mortality rate, which was enhanced to 85.71% by the addition of xanthan 
gum (NF2). Environmentally friendly synthetic compounds also showed high toxicity to the 
target pest. Importantly, neither the essential oil formulations nor the synthetic compounds 
exhibited phytotoxicity to lettuce seedlings. Furthermore, the contact angle measurements for 
the oil formulations ranged from 22° to 32°, indicating favorable spreading potential on 
surfaces.
This research provides evidence supporting the potential of both enhanced essential oil 
formulations and environmentally friendly synthetic compounds as effective and safe 
alternatives to conventional pesticides, offering valuable insights for developing sustainable 
pest control strategies.The study identified two promising paths for environmentally friendly 
pest control. First, a peppermint oil formulation enhanced with xanthan gum (NF2) 
demonstrated superior insecticidal efficacy against Tribolium castaneum larvae, achieving a 
mortality rate of approximately 85.71%. Second, environmentally friendly synthetic 
compounds proved highly effective, with mortality rates reaching 100%. The best formulation 
was the xanthan gum (SF2) enhanced formulation, which demonstrated high efficacy and better 
dispersal, demonstrating its strong potential as an alternative to conventional insecticides.
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masters, 2025. DEPARTMENT OF BIOLOGY . Faculty of Nature and Life Sciences. University of El-Oued.