المساھمة في دراسة نواتج الأیض الثانوي و الفعالیة البیولوجیة لمستخلصات نبات القزاح : Pituranthos chloranthus

Abstract

This study aimed to investigate the outputs of secondary metabolism and determine the biological effectiveness of Pituranthos Chloranthus' extracts. In order to achieve this goal, we first used the chemical examination, which showed the presence of most secondary metabolites. next, Preparation of methanol, ethanol and aqueous extracts by soaking and the extraction of essential oil through hydro-distillation. In turn, the yield of each extract has been recorded; the highest percentage has been associated with the methanolic extract at 5%, followed by 4.5% that of the aqueous extract, and the ethanolic extract by 3.46%, the yield recorded in essential oil was 0.4%. The plant's moisture content was estimated at 43.8%. Secondly, phenol, flavonoids and tannins were estimated in aqueous, methanolic and ethanolic extracts. The highest poly phenol content was found in the methanolic extract (14.65± 2.33 mg AG E/g MS), while the lowest value spotted in aqueous extract (11.22±0.98 mg AG E/g MS). The quantative estimate of flavonoids was as its highest value in methanolic extract at an estimate of (2.9885±0.035 mg Q E/g MS) and the lowest value was found in aqueous extract (0.7494±0.013 mg Q E/g MS) , whereas the highest value concerning tannins was spotted in ethanolic extract with an estimate of (5.31±0.16 mg CA E/g MS) and its lowest value in aqueous extract (4.04±0.30mg CA E/g MS) Thirdly, in terms of biological efficiency, the aqueous, methanolic and ethanolic extracts showed a moderate antioxidant activity where the IC50 was estimated at 0.54-0.57-0.59 mg/ml respectively, while the essential oil antioxidant activity was low and IC50 estimated to be 4.49 mg/ml . the antibacterial activity for four bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus) was positive only with the pure essential oil and was not with the rest of the extracts, and the best diameter inhibition was recorded at the 100% concentration with the value of 14.9 mm in S. aureus .