HighPerformanceLiquid Chromatography of antioxidant activity of seeds some varieties of prickly pear (Opuntia ficus-indica L.) from the Sidi-Fredj Souk Ahras Algeria
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Date
2022-06-30
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جامعة الوادي - University of Eloued
Abstract
The study was conducted on seeds of three varieties of Opuntia ficus-indica (O. ficus-indica),
harvested from different regions of Souk Ahras in north-East of Algeria (Sidi-Fredj, Taoura
and Drea). The analysis consisted of antioxidants extraction, following the solid-liquid
extraction with ethanol 40% (v/v), their quantification and determination of their antioxidant
activity by two methods (reducing power and DPPH (2-2 diphenyl 1-picryl hydrazyl) Test).
Results show that total phenolic content (TPC) ranged from 71,01to 90,79mg GAE (Gallic
Acid Equivalent)/100g Extract. The Sidi-Fredj variety has the best rate followed by the Taoura
and Drea ones. Concerning, flavonoids and tannins, the Taoura variety contains higher
concentrations corresponding to 2.67 mg QE (Quercetin equivalent)/100g and 6.60mg CE
(Cyanidin equivalent)/100g. Activities of extracts of three varieties show similar performance;
the EC50 for the reduction of ferric iron 0.05g/mL for all extracts, whereas the EA is more
important in Sidi-Fredj and Drea seeds extracts. Regarding the scavenging of DPPH, Drea and
Sidi-Fredj varieties show the highest capacity equals to 0.20g/mL. Linear correlations between
the different studied activities and some antioxidants (flavonoids, tannins) rates were noticed,
indicating their participation in the obtained effect.
Analysis of the oil by HPLC revealed the presence of phenolic compounds including gallic
acid and a form of vitamin E (α-tocophérol), capable of expressing biological activities; Antioxidants and vitamins. GC (Gas Chromatography) analysis showed that prickly pear oil was a
major source of essential fatty acids (C18:2 and C18:3). The anti-free radical activity of the
seed was evaluated through a DPPH chemical test. The results obtained show a hight reducing
activity of our extract.
Description
article
Keywords
Opuntia ficus-indica, Seeds, Oil, Antioxidant, Anti-free radical activity
Citation
.Abderrahmane, BOUAOUICH , Faiza, Bouguerche . HighPerformanceLiquid Chromatography of antioxidant activity of seeds some varieties of prickly pear (Opuntia ficus-indica L.) from the Sidi-Fredj Souk Ahras Algeria . Algerian Journal of Biosciences . Vol 03. N 01. 30/06/2022. University of Eloued .[Write down the date][Copy the download link ]