AJP_Vol 02 N 02
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Item Ethnobotanical investigation and Morphobiometric characterization of different cowpea seeds (vigna unguiculata subsp. unguiculata (L.) walp.) in the Hoggar region (Algerian Sahara): acquisition and future investment project for food security in Algeria.(جامعة الوادي - University of Eloued, 2021-12-30) Aida, BASSEDDIK; Sihem, TELLAHThis survey was conducted to establish an inventory of cowpea culture in the arid region of southern Algeria in 2018. To this end, missions were organized to collect accessions, provide information on cultural practices and traditional knowledge related to this culture a semi-questionnaire The European Commission has set up a European Agricultural Information Society. The study found that among the farmers surveyed. This study showed that of the farmers surveyed, 70.02% were men and 29.07% were women. Farmers cultivate cowpea on small areas, its production is used only for own consumption and a small one is marketed in villages where the plant is grown, the farmers interviewed use three main parts: seeds, green pods and hay. After harvest, the rest of the plant (leaves and stems) is used to feed livestock (sheep and cattle). Cowpea cultivation is in danger of extinction, according to the majority of farmers surveyed, diseases and insect attacks are low (32%). In addition, low rainfall, electricity and labor shortages during manual harvesting appear to be the main production constraints reported by the farmers interviewed. While farmers are satisfied with the yields obtained. The results obtained by biometric morpho characterization showed a wide phenotypic variability of the seeds However, significant differences were detected in several morphological characters.Item Comparative Chiral Separation of (RS)‐Propranolol Racemate by HPLC Using α-Glycoprotein and β-Cyclodextrin Stationary Phases.(جامعة الوادي - University of Eloued, 2021-12-30) Derouicha, MATMOUR; Fatima Zohra, HADJADJ AOUL; Yassine, MERAD; Nazim, BELLIFA; Hassen, GUERMOUCHEThe most of the β-blockers are still clinically being sold as a racemic mixture despite the fact that their enantiomers show significant differences in the pharmacological effects and activities. This paper describes a comparative study of tow chiral separations of (RS)‐Propranolol racemate by HPLC using α-Glycoprotein (AGP) and β-Cyclodextrin (BCD) Stationary Phases. For the AGP separation, the column size was (150 mm X4 mm X 5 μm), the mobile phase composed of Propanol-2 and Ammonium acetate (0.5:99.5 v/v), at a flow rate of 0.9 mL/min and the detection by ultraviolet absorption at 225 nm. For the BCD separation, the column size was (200 mm X4 mm X 5 μm), the mobile phase composed of Acetonitrile: Ethanol: Acetic acid: Triethylamine (960: 33: 4: 3 v/v/v/v), at a flow rate of 1 mL/min and the detection by ultraviolet absorption at 225 nm. The retention time of SPropranolol and R-Propranolol with AGP separation was respectively: 7.25 min and 11.82 min while with the BCD separation 16.18 min and 18.50 min respectively. The racemate contains 50.46 % of S-Propranolol and 49.53 % of R-Propranolol with AGP separation while with BCD separation, it contains 50.43 % of S- enantiomer and 49.57 % of R-enantiomer. There is a similarity between the enantiomeric purity values and the enantiomeric excess values of tow separations, but the separation with AGP stationary phase is faster than with the BCD stationary phase. For a selective β-blocking use, it could be very interesting to encourage its production in its form enantiomerically pure wich is the S-enantiomer.Item The medicinal plants of Bousaada (M’sila, Algeria)(جامعة الوادي - University of Eloued, 2021-12-30) MARROUCHE, Hinda; BACHIRI, Missoune; BOUNAR, RabahThe study area of Bousaada is characterized by a rather remarkable flora, the floristic list of identified plants is 88 species belonging to 82 genera and 43 botanical families. These plants are of ecological, medical and economic interest which must be preserved, conserved, to ensure their sustainability. The method of approach is an ethnobotanical survey carried out in the Bousaada region, which was chosen for its florist, ecological and climatic diversity and offers the local population a fairly rich knowledge of traditional herbal medicine, and the fact that traditional healers are known to have a good knowledge of the use of medicinal plants. This ethnobotanical study is carried out using a questionnaire of the survey is divided into two parts making it possible to collect information on the person (sex, age, levels of study, family situation), and on medicinal plants (name, use, part used, method of preparation). The aim of this study was to identify, catalog, document the large number of medicinal plants used in the region of Bousaada for the treatment of various human pathologies, and how to exploit and conserve this rich floraItem Diversity and distribution patterns of reptiles in the northern Algerian Sahara (Oued Souf, Taibet and Touggourt)(جامعة الوادي - University of Eloued, 2021-12-30) Aicha, MOUANE; Abdel Karim, HARROUCH; Ismail, GHENNOUM; Makhlouf, SEKOURThe present study was based on the observations and captures of reptiles in the Northern Algerian Sahara (2011-2013). The studied habitats were the erg (sand dunes), palm grove, reg, Sebkha, and urban sites. We have identified 30 species (20 Saurians, 9 Ophidians and one Testudines) these species are classified into two orders (Anura and Squamata), 12 families and 22 different genera. Among the identified species, 7 protected species in Algeria and 5 species endemic to the Mediterranean. A total of 93.3% (28 species) are assessed as Least Concern and one species was considered to be Data Deficient (Scincopus fasciatus). One species is listed as near threatened (Uromastix acanthinurus). The highest species richness was noticed in the palm groves with 19 species as well as the Shannon diversity index= 3.35. The evenness is at its highest in the sebkha and the urban site with 0.9 and 0.86. The correspondence factorial analysis showed that some species were characteristic of habitats such as Tarentola neglecta and Tarentola deserti which are a specific species of the urban sites and the palm groves, while Scincus scincus was a specific species of the Erg. The generalized linear model showed that the geographic coordinate, spatiotemporal factors and human activity have significant effect on the distribution of reptiles.Item In-vitro Antioxidant activity, Biosafety, Nociceptive and Antiinflammatory potential of Acetone Polyherbal (ELNA) Extract in mice(جامعة الوادي - University of Eloued, 2021-12-30) MacDonald, Idu; Benjamin, Ogunma Gabriel; Chike Uzochukwu, OnuigboELNA (Moringa oleifera, Crateva religiosa and Curcuma longa) is a polyherbal formulation comprising of Moringa oleifera, Crateva religiosa and Curcuma longa. It is used traditionally in the treatment of inflammation, gastro-intestinal infections, hypertension and immune compromised diseases. This study investigate the in-vitro anti-oxidant capacity, biosafety, nociceptive and anti-inflammatory potential of polyherbal ELNA acetone extract. In-vitro antioxidant study was done on the extract using a standard protocol of 1, 1, Diphenyl 2, picrylhydraxyl (DPPH) radical scavenging activity. Toxicological profiling was done using standard methods. Acetic acid, hot plate and egg albumin models were designed for the nociceptive and anti-inflammatory properties of the extract. DPPH scavenging property of the extract showed significant increase at graded concentration. Acute toxicity study of ELNA acetone extract revealed no toxic effect, with absent mortality and less adverse effect (LD50>5000 mg/kg body weight). The sub-chronic toxicity study of the acetone extract for 28 days, showed no significant difference (p= 0.05) in the organ to body weight ratio. The haematological indexes indicated no significant different except at 400 mg/kg that elicited slight significant (p<0.05) increase in the platelet. The results showed that graded doses of the extract at (400, 800 and 1200 mg/kg body weight) exhibited significant (p<0.05) decrease in peripheral and central pain also a decrease in the paw edema volume of inflammation. This was achieved in dose dependent manner. The result of this study established a pharmacological evidence for the traditional use of ELNA as an analgesic and anti-inflammatory agent, it also present information on the anti-oxidant properties and toxicity profile of the formulation.Item Phytochemistry, In-vitro antioxidant, Microbicidal, Anti-ulcerogenic and Biosafety Potential of Emilia coccinea Aqueous Extract in Animal models.(جامعة الوادي - University of Eloued, 2021-12-30) MacDonald, Idu; Benjamin Ogunma, Gabriel; Charles Osayande, AgboonogievaEmilia coccinea enhances the treatment of several disease conditions include; vertigo management, ringworm, cough, gonorrhoea, ulcers, lice, measles, seizure, eye drop. This study investigates the phytochemistry, In-vitro antioxidant, anti-ulcerogenic, microbicidal and biosafety effect of aqueous extracts of E. coccinea using animal model. E. coccinea leaf was freshly obtained, shade dried, pulverized and prepared into aqueous extract. Standard procedure were used for the evaluation of the phytochemicals, in-vitro antioxidant and antimicrobial activities. Twenty five (25) Wistar rats were acclimitized and randomly selected into five groups (n=5) such as untreated group, 10 mg/kg cimetidine and graded doses (100, 200 and 400 mg/kg b.w.) of aqueous extract of E. coccinea to evaluate antiulcer and twenty Wistar rats were used for the biosafety effects. Results from the qualitative and quantitative phytochemical screening showed the present of phenol (47.19 mg), saponins (84.64 mg), alkaloids (75.17 mg), cardiac glycosides (63.12 mg) and anthraquinone (48.79 mg). The antioxidant property showed a competitive scavenging effect against 1,1 diphenyl2-picrylhydraxyl radical when compared with ascorbic acids. Microbial activities of the aqueous extract of E. coccinea at various concentration elicited inhibitory effect against Salmonella typhii, Escherichia coli, Helicobacter pyloris. The ulceration in rat stomach lining induced with 70% ethanol and pretreated with prophylactic measure of aqueous crude extract showed significant reduction in ulcer count, ulcer index with increased percentage inhibition of ulcer when compared with untreated and reference control. The haematological and histopathological study shows no significant difference in the aqueous crude extract with absent toxicity when comparisons with the control