Browsing by Author "Lanez, E"

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    IN VITRO AND IN SILICO EVALUATION OF ANTICANCER ACTIVITY OF N,N-DIMETHYLAMINOMETHYLFERROCENE
    (university of eloued -جامعة الوادي, 2019-05-01) Adaika, A; Lanez, T; Lanez, E
    The aim of this study was to examine the interaction of DNA with the ligand N,N-dimethylaminomethylferrocene(NDF) in buffer phosphate solution(pH = 7.2) by electronic spectroscopy, electrochemical and molecular docking technics. The electrochemical results showed that the binding constant and free binding energy of the adduct DNA-NDF is equal to 1.48×10-4and -23,81 KJ.mol-1respectively. These results were in good agreement with those obtained from electronic spectroscopie assays;furthermore the interaction was also confirmed by the low value of diffusion coefficient of the bounded ligand1.83×10-8cm2.s-1as compared to the free ligand 2.13×10-8cm2.s-1. In addition the in silico study by molecular docking confirms the obtained in vitroresults. Molecular docking further indicated that the ligand is binds to DNA via a hydrogen bond betweenitsnitrogen atom and the oxygen number 4 of the deoxyadenine of the DNA B-chain
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    INVESTIGATIONS OF THE BINDING PARAMETRES OF THE INTERACTION OF N'-FERROCENYLMETHYL-N'-PHENYLACETO- AND PROPIONOHYDRAZIDE WITH DNA
    (university of eloued -جامعة الوادي, 2019-05-01) Mouada, H; Lanez, T; Lanez, E
    Herein we describe the determination of binding parameters of the interaction of N'- ferrocenylmethyl-N'-phenylacetohydrazide (FcA) and N'-ferrocenylmethyl-N'-phenylpropionohydrazide (FcP) derivatives with chicken blood double-stranded DNA (cb-ds DNA) in phosphate buffer (PB) solution at physiological pH 7.2 by electronic spectroscopy and molecular docking calculations. The results indicated that both FcA and FcP bind DNA in groove binding mode, values of binding constants and binding free energy obtained from electronic spectroscopy measurements and molecular docking calculations were in good agreement