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Item Morphological and anatomical comparison of leaves of date palm cultivars (Phoenix dactylifera L) grown in the southeastern region (Algeria)(University of Eloued جامعة الوادي, 2023-10-22) Laouedj, Hacene; Kheraze, Khaled; Ghemam Amara, Djilani; Keddour, AbdelbasetThis study was carried out in the laboratory of the Faculty of Natural and Life Sciences at the University of ElOued. Where this work aims to study the anatomical comparison for leaves from the date palm of five varieties (Garess, Hamraya, Deggla Bidha, Deggla Nour, Tekermist) and cultivated in the area EL-oued, Through a morphological description (Length and width of the frond, yield) and anatomical study (the diameter of the leaf, Diameter of the large, medium and small vascular bundle, the diameter of the large fibrous bundle, the number of fibrous bundle between the two large bundles, the number of small and medium vascular bundles between the two large bundles, The area of the primary and secondary wood in the large beam, the thickness of the epidermis and waxy layer and the middle tissue). Through the results obtained, it was found that the Hamraya variety achieved the highest yield in the number of inflorescences per palm tree, This variety (Hamraya) was characterized by the largest number of medium vascular bundles compared to other varieties, It was also characterized by the smallest thickness of the waxy layer, the diameter of the medium vascular bundle as well as the diameter of the large fibrous bundle, As for the rest of the studied characteristics, they differed between the largest and the smallest compared to the other varieties. نفذت هذه الدراسة في مخبر كلية العلوم الطبيعة والحياة في جامعة الشهيد حمه لخضر بالوادي للسنة الجامعية 2021/2022. حيث يهدف هذا العمل الى دراسة المقارنة التشريحية لأوراق من نخيل التمر لخمسة أصناف (غرس، حمراية، دقلة بيضاء، دقلة نور، تكرمست) والمزروعة في منطقة الوادي (حاسي خليفة)، من خلال الوصف المرفولوجي (طول و عرض السعفة، المردود ) والدراسة التشريحية (قطر مقطع الورقة، قطر الحزمة الوعائية الكبيرة، متوسطة وصغيرة، قطر الحزمة الليفية الكبيرة، عدد الحزم الليفية بين الحزمتين الكبيرتين، عدد الحزم الوعائية الصغيرة والمتوسطة بين الحزمتين الكبيرتين، مساحة الخشب الأولي والثانوي في الحزمة الكبيرة، سمك البشرة والطبقة الشمعية والنسيج الوسطي). من خلال النتائج المتحصل عليها تبين أن صنف حمراية حقق أكثر مردود من خلال عدد النويرات في النخلة، حيث تميز هذا الصنف (حمراية) بأكبر عدد في الحزم الوعائية المتوسطة مقارنة بالأصناف الأخرى، كما تميزت بأصغر سمك للطبقة الشمعية، قطر الحزمة الوعائية المتوسطة وكذلك في قطر الحزمة الليفية الكبيرة، أما في باقي الصفات فكانت متوسطة مقارنة بالأصناف الأخرى.Item Current Review on Herbal Pharmaceutical improve immune responses against COVID-19 infection(A&V Publications, 2020-07-01) Derouiche, SamirCoronavirus SARS-COV2 (COVID-19) represents the causative agent of a potentially fatal disease that is of great global public health concern. The pandemic outbreak of COVID-19 is rapidly spreading all over the world. This review aims to provide the role of Herbal Pharmaceutical in reducing the spread or complications of COVID-19 infection in the body of people. The evidence of generation of oxygen free radicals and oxidative stress is a key process in the onset of chronic disease which participate in the development of the systemic inflammatory response syndrome. Medicines of plant origin aroused a lot of interest due to their wide use, abundance, high efficacy and lack of secondary effects as an antioxidants and anti-inflammatory agents, antiviral efficacy or in their preventive efficacy of the immune system From it we suggest that the combination of conventional therapy and herbal medicine for patients infected with COVID-19 to reduce the complexities and development of the virusItem EFFECT OF EXTRACTS AQUEOUS OF PHRAGMITES AUSTRALIS ON CARBOHYDRATE METABOLISM, SOME ENZYME ACTIVITIES AND PANCREATIC ISLET TISSUE IN ALLOXAN-INDUCED DIABETIC RATS(International Journal of Pharmacy and Pharmaceutical Sciences, 2017-06) Derouiche, SAMIR; Azzi, Manel; Hamida, AbirObjective: The present study was aimed to investigate the effects of the aqueous extract of rhizome of phragmites australis on carbohydrate metabolism, some enzyme activities and pancreatic tissue in alloxan induced diabetic rats. Methods: Wistar rats were divided into three groups (n=6) as control, diabetic group and test groups (Diabetic+AEPA). Diabetes in rats was induced by alloxane using a single peritoneal injection of 150 mg/kg dose. Aqueous extract of Phragmites australis was supplemented (200 mg/kg b. w) orally for three weeks. The aqueous extract of Phragmites australis was prepared and phytochemical were analyzed by using standard methods. Blood glucose level, pancreas histology and various biochemical parameters were assessed. Results: The results of the phytochemical analysis of aqueous extract of Phragmites australis (AEPA) revealed the presence of tannin, terpenoids, glycosides and flavonoids. Compared with the control, a significant decrease in the body weight gain (p<0.01) and increase in food intake (p<0.001) were noticed in the diabetic group. The biochemical evaluation showed significantly higher values for glucose (p<0.001), lipid profile (p<0.05), transaminases (p<0.05), amylase and alkaline phosphatase (p<0.001) activities in diabetic group compared with the control. Histology of the pancreas showed congestion of vessels and focal area of necrosis in diabetic untreated rats. However, treatment with aqueous extract of Phragmites australis significantly normalized blood glucose, serum biochemical profile and pancreas histomorphology. Conclusion: It is suggested that aqueous extract of Phragmites australis exhibits a benefic effect in rat to a great extent in attenuating and restoring the damage sustained by diabetesItem Zinc Supplementation Overcomes Effects of Copper on Zinc Status, Carbohydrate Metabolism and Some Enzyme Activities in Diabetic and Nondiabetic Rats(Elsevier, 2016-08-01) DEROUICHE, Samir; Kechrid, ZineObjective: The aim of this study was to investigate the effect of zinc supplementation on zinc status, carbohydrate metabolism and some enzyme activities in rats with alloxan-induced diabetes that were fed high-copper feed. Methods: Male albino Wistar rats were randomly divided into 6 groups (n=10). The first and fourth groups were nondiabetic and diabetic controls. The second, third, fifth and sixth groups were copper, copper + zinc, diabetes + copper and diabetes + copper + zinc groups, respectively. Diabetes in the fourth, fifth and sixth groups was induced by alloxan. Copper (30 mg/kg feed) as CuSO4 5H2O and zinc (231 mg/kg feed) as ZnSO4 7H2O were added to the feed of the animals in the copper and zinc groups for 21 days. Results: Copper supplementation caused a significant decrease in body weight gain, serum zinc, tissue zinc, serum protein concentrations, alkaline phosphatase, lactic dehydrogenase and amylase activities. In contrast, it led to an augmentation in creatinine, uric acid and transaminases activities in rats with and without diabetes. Zinc supplementation in the feed for animals given copper ensured a partial correction of the previous parameters. Conclusions: The study demonstrated the beneficial effects of zinc treatment in copper-induced metabolic disturbance in diabetic and nondiabetic ratsItem STUDY OF FLUORIDE-INDUCED HAEMATOLOGICAL ALTERATIONS AND LIVER OXIDATIVE STRESS IN RATS(WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, 2017-05) Djouadi, Anfal; Derouiche, SamirFluorine is a necessary biological trace element for human health. However, fluoride accumulation leads to cascading effects resulting in altered physiological functions in human being. The current studies evaluated the effect of fluoride induced hematological alterations and oxidative stress in the liver of experimental rats. For this purpose, adult female Wistar albino rats divided into control and NaF treated group. sodium Fluoride (400 mg/kg b.w) added in their drinking water for 70 days. Compared with the control, a significant decrease in the body weight gain (p<0.001) and increase in relative liver weight (p<0.001) were noticed in the NaF group Our results showed that fluoride treatment caused a increase in Granulocyte (p < 0.001), White blood cell (p < 0.001), Lymphocyte (p < 0.01) and Platelets (p < 0.01) compared with the control. In addition, it led to an increases in triglyceride (p<0.001), cholesterol (p<0.01) level, GOT (p<0.01) and GPT (p<0.001) activities in serum animal compared to the control group . In contrast fluoride caused an oxidative stress by decrease in GSH level and GST activity and increase in MDA concentration compared with the control group. In Conclusion, Results demonstrated the toxic effect of high-dose of fluoride by causing oxidative stress and inflammation in hepatic tissue.Item Biological properties and Acute Toxicity Study of Copper oxide nanoparticles prepared by aqueous leaves extract of Portulaca oleracea(A & V Publications, 2020-06-03) Atoussi, Ouidad; Chetehouna, Sara; Derouiche, SamirThe aim of this study is to use the purslan (Portulacae oleracea L) leaves aqueous extract in order to prepare copper nanoparticles and to explore antioxidant and anti-inflammatory activity of aqueous extract of P.oleracea L and CuNPs. The nanoparticles were characterized from structural and morphological point of view by using analytical techniques such as: UV-Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). Antioxidant activity of purslane and CuNPs was studied done FRAP assay also the anti-inflammatory and hemolysis essay were studies. The acute toxicity test was applied in Wistar albino rats. Obtain results show that the SEM analysis revealed that the particle size was found to be ranging under 46 nm. On the other hand, the copper nanoparticles and purslan possessed the reducing capacity when IC50 value was 68, 316 μg/ml and 79, 675 μg/ml respectively, the anti-inflammatory ability while IC50 value was 77, 503 μg/ml and 60, 727 μg/ml respectively. In this study, the toxicity test showed no mortality or behavioral change up to 20 mg/kg of albino Wistar rats CuNPs. We concluded that Portulaca oleracea L has potential properties as biocatalyst or natural stabilizers for CuNPs synthesis. Each CuNPs as P.oleracea L leaves extract were have a reducing and anti-inflammatory capacity which can be protect the cells against the degenerative effects of Reactive Oxygen Species (ROS).Item Study of oxidative stress during pregnancy(Juniper Publishers, 2017-10-10) Derouiche, Samir; Doudi, Dalal; Atia, NouraOxidative stress is defined as an imbalance in the balance between antioxidants and pro-oxidants in favor of antioxidants. The increase in lipid peroxidation and the significant decrease in GSH and total antioxidant power ORAC in the serum and erythrocytes of pregnant women especially during the third trimester of pregnancy clearly show the evolution of the state of stress oxidative therapy associated with pregnancy in pregnant women. So pregnancy is a physiological state characterized by oxidative disturbance that contributes to the initiation and progression of complications associated with pregnancyItem Oxidative Stress Associated with SARS-Cov-2 (COVID-19) Increases the Severity of the Lung Disease - A Systematic Review(Journal of Infectious Diseases and Epidemiology, 2020-05-04) Derouiche, SamirCOVID-19 patients have a higher risk of developing inflammatory responses associated with serious and even fatal respiratory diseases. This review focuses on the relationship between oxidative stress and COVID-19. Coronaviruses are a family of common RNA viruses that can cause serious lower respiratory tract infections, followed by bronchitis and pneumonia. Pulmonary inflammation, fever and fibrosis are symptoms of COVID-19 mediated by cytokine pro-inflammatory. Oxidative stress affect repair mechanisms and the immune control system, which is one of the main events of the inflammatory response which allows us also to conclude that oxidative stress is a major factor increasing the severity of COVID-19 especially during chronic diseases associated with the fragility of the antioxidant system, suggesting to recommend antioxidants supplementation in therapeutic strategies against COVID-19.Item Bio-activity of essential oils from Cymbopogon schoenanthus L(University of Eloued جامعة الوادي, 2021-10-24) KADRI, Mounira; BELBOUT, Amel; SALHI, Nasrine; YAHIA, Abedelouahabالفعالية البيولوجية للزيت الأساسي للماد Cymbopogon schoenanthus L تهدف هذه الدراسة إلى التحقق من الفعالية المضادة للأكسدة و الفعالية ضد بكتيرية للزيت الأساسي لنبات اللماد النامي في الصحراء الجزائرية تم استخلاص الزيت الأساسي للجزء الخضري للماد عن طريق التقطير المائي (كليفنجر) لمدة 3 ساعات. أما الفعالية البكتيرية للزيت الأساسي للماد فاستعملنا طريقة الانتشار عبر الأقراص في وسط الجيلوز . السلالات البكتيرية المختبرة هي .Klebsiella pneumoniae ATCC 700603, Listeria innocua Clip74915, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella enterica ssp. Arizonae CIP 81, Staphylococcus aureus ATCC 25923, Bacillus cereus, Enterococcus faecalis الفعالية المضادة للأكسدة تم اختبارها عن طريق قدرة الزيت على كبح الجذر الحر للDPPH. نبات اللماد غني بالزيت الأساسي و الذي قدر مردوده بـ 6.06±0.54 % كما قدرت قيمة IC50 في الفعالة المضادة للأكسدة ب28.82±11.72 mg/ml لم يكن للزيت الأاسي تأثير واضح على السلالت البكتيرية Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella enterica ssp. Arizonae CIP 81, Bacillus cereus, Enterococcus faecalis،لكنه اضهر فعالية ضد Klebsiella pneumoniae, Listeria innocua, Staphylococcus aureus،و أعلى قطر تثبيط رسم عند السلالة Klebsiella pneumoniae,بـ 36.67±1.53mm. Bio-activity of essential oils from Cymbopogon schoenanthus L. This study aimed at investigating the Antioxidant and antibacterial activity of essential oils from Cymbopogon schoenanthus L. (spreng) was collected of south Algeria. The essential oils were extracted by hydrodistillation of fresh aerial part of C. schoenanthus a (Clevenger-type apparatus) for 3 h. The antimicrobial activity of C. schoenanthus essential oil was evaluated using Agar well-diffusion test (Lopes-Lutz et al., 2008). The microorganisms tested were Klebsiella pneumoniae ATCC 700603, Listeria innocua Clip74915, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella enterica ssp. Arizonae CIP 81, Staphylococcus aureus ATCC 25923, Bacillus cereus, Enterococcus faecalis. The antioxidant and free radical scavenging activity were determined by spectrophotome, Based on DPPH hydroxyl radical scavenging activity. The Cymbopogon schoenanthus was rich in essential oil, yielding (6,06±0,54%). Antioxidant activity essential oil has been done by using DPPH essay. IC50 values observed for DPPH essay were 28,82±11,72 mg/ml. The essential oil was not effective against Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella enterica ssp. Arizonae CIP 81, Bacillus cereus, Enterococcus faecalis. The essential oil of C. schoenanthus has an antibacterial effect against, Klebsiella pneumoniae, Listeria innocua, Staphylococcus aureus, the Highest inhibition was noted against Klebsiella pneumoniae (36,67±1,53mm).Item The Effect of natural organic manure on the growth and productivity ofpotato (solanum tuberosum Z var spunta) in a Sahara desert region(University of Eloued جامعة الوادي, 2013-12-17) Ghemam Amarad, D.; Senoussi, M. M.Many studies have been carried out on the growth, productivity and nutritional properties of potato, including the effects of organic manures on tuber yield and quality. However, there are few studies on the effect of organic manure on potatoes agriculture in arid zones. The main objective of this study was to investigate the effects of natural organic manure on the growth and productivity of potato (Solanum tuberosum, cv. Spunta) in a Saharian region (El-oued, Algeria). An experiment was laid out out in a randomized complete block design (RCBD), with four replications and five labeled treatments, the control (no manure), the common fertilization treatment applied by the potatoes farmers 2 q/h (15-15-15 NPK) + 25 t/h poultry manure, 50 t/h of poultry manure, sheep manure and 50 t/h of mixed manure poulty and sheep 1:1 w/w. Results showed an increase in the foliage area, plant cover area and the number of stems per plants by adding poultry manure, and mixed manure more treatments of mixed manure (poultry and NPK) or sheep manure in comparison with control (no manure). The treatment by mixed manure showed significant increase in the number of tubers per plant in comparison with other treatments, but all the used treaments caused a significant increase in the tuber weights and the quantity of production and the percent of standard tubers in comparison with control. The treatments with poultry manure gave the hihest yied in increasing the yield with high significant difference incomparison to other treatments. Thus, the combination of natural organic manures and low rates of inorganic fertilizers is a promising low cost option in production of high yields of potatoes.Item Composition chimique et évaluation des activités biologiques de la partie aérienne d,une plante endémique Myrtus nivellei(University of eloued جامعة الوادي, 2017-10-05) Ramdane, Farah; Mahfoud, Hadj Mahammed; Ould Hadj Mohamed, DidiLes substances naturelles issues des végétaux ont des intérêts multiples mis à profit dans l'industrie: en alimentation, en cosmétologie et en dermopharmacie. Parmi ces composés on retrouve dans une grande mesure les métabolites secondaires qui se sont surtout illustrés en thérapeutique. Selon lalittérature, les composés d'origine naturelle présentent I'avantage d'une très grande diversité de structures chimiques et ils possèdent aussi un très large éventail d'activités biologiques, dans le but d'avoir des nouvelles ressources bioactive; nous avons réalisé une étude ethnobotanique dans le Hoggar afin de connaitre l'utilisation de cette plante dans la médecine populaire, ensuite Les constituants des huiles essentielles des parties aériennes de cette plante extraites par hydrodistillation ont été analysés par GC-MS. Cette analyse fait apparaître 24 composés représentant 98,87yo de I'huile total. Le 1,8 cineole (53,44%) est le composé majoritaire de cette huile suivi par 12,2, 6,7-tetramethyl-10- oxatricyclo 14.3.0.1(1,7)]decan-5-one (ll,73Yo), trans ocimene (7,54yo),linalyl propirionate (6,8t%) et lavandulyl acetate (5,24%). Cette huile a montré une activité importante contre le radical DPPH. L'activité antibactérienne de cette huile a été déterminée par la méthode de diffusion sur des souches Gram positif et d'autres Gram négatif, cette huile n'a pas montré une efficacité que sur la souche testée Listeria monocytogenes. Egalement l'huile de cette espèce a inhibé modérément la charge parasitaire testée particulièrement la souche Leishmania majorItem ETUDE PHYTOCHIMQUE ET BIOLGIQUE D'EXTRAIT METHANOLIQUE DU ZIZYPHUS LOTUS L.(University of eloued جامعة الوادي, 2017-10-05) Kadri, Mounira; Salhi, Nasrine; Abdelouahad, Yahia; Sahraoui, Nada; Selami, AmelItem Effect of some medicinal plant extracts to combat disease worm (Ectomyelois ceratoniaezeller) in the date Deglet Nour (phoenix dactyliieraL.)(University of eloued جامعة الوادي, 2017-10-05) Hamada, Samra; BelhamraThe varietyDeglet NourPhoenx dactytifera is the most famous and exporters date with an annual production of 26.5 million q/years. Because of the spread of some diseases and pests, especially worm dates Ectomyelois ceratoniae, as well as birds are harmful to its production marvelous is lost annually between 2O% to 30%, therefore the objective of this research, comes within the framework of agricultural development of wealth fruit of the date palms, and developing comprehensive strategy to combat diseases tree date palm. Applications of modern research appeared to àontrol crop pestJând vegetables, using of bioactive natural products as herbals, to protect agricultural production and human health. The first step of this research is the identification of medic]nal plants collected, and selected of more than 20 plants, and to know the nature of the Principe activé ingredients in each plant'As for the effect-of some extracts applied, we find an influence effectively à percentage the death of the worm: Alllum sativum (peel and cloves of garlic),Artemisia herbà aba, i-hapsia garganica, Teucrium polium, Euphorbia peptus, Solanum tuberosum, Papaver somniferum, punica gratum, and Camellia slnensi. The tested plant extracts are efficacy to combat against a disease worm dates Ecfomyelois ceratoniae, and we can use them as insecticideô.Item Effect of some agricultural residues on the characteristics of growth and yield of the onion bulb (Allium cepa L)(University of eloued جامعة الوادي, 2019-04-15) Ghemam Amara, Djilani; Rebiai, Abdelkrim; Alia, Zaid; Djihad, Saoud; Kherraz, Khaled; Mesbahi, Mohammed Adel; Chemsa, Ahmed Elkhalifa; Senoussi, Mohammad Mouradthe study was conducted at the primary school farm in Rabbah zone during in 2016/2017 season, To evaluate the contribution of fram residues (palm residues, Peanut Peels, cooked tea leaves) in vegetative growth and the bulb onion in addition to the total yield of onion plant. An investigation was a randomized complete block design in three replications. The obtained results of experiment showed that: - Application of fertilize by cooked tea leaves were significantly better in of the studied characters (plants height and Leaves Area) with rate increased to 47.15% and 93.4% respectively, than control treatment. Followed by T1 and then T3. - Peanut Peels treatment gave the largest number of tubular blades in the plant(12.33), the diameter of the bulb neck(13.48mm) and bulb Length(7.35cm) than other treatments. Followed by T2 and then T3. -cooked tea leaves plants gave highest diameter of bulb(5.42cm), highest bulb weight(66g) and yield(20.85 t/ha) in the season. where Achieving with T1(18.35t/ha) significantly increase in the yield total in comparison with other treatments.