Comparison Of Protein Extraction Methods For The Leaves Of Ficus Deltoidea

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dc.contributor.authorAbdullah, F. I.
dc.contributor.authorChua, L. S.
dc.contributor.authorRahmat, Z.
dc.date.accessioned2020-11-05T09:27:24Z
dc.date.available2020-11-05T09:27:24Z
dc.date.issued2017-05-01
dc.descriptionArticale in Journal of fundamental and Applied Sciences Vol. 09, N. 02en_US
dc.description.abstractThis study investigated several extraction methods for proteins from the leaves of Ficus deltoidea. The protocols include solvent based extraction, TCA-acetone precipitation, Tris buffered phenol extraction and hybrid technique of TCA-acetone/phenol-SDS. The results indicated that the hybrid technique and Tris buffered phenol method could produce higher number and better quality of proteins. There are 22 protein bands with the wide range of molecular size ranging from 8.20 to 113.48 kDa separated by 12% polyacrylamide gel electrophoresis in the hybrid technique. Tris buffered phenol could extract 13 protein bands from the plant, but only 9 protein bands from TCA-acetone precipitation method. pH 8.0 was the optimum value of Tris buffered phenol for protein extraction with higher protein content and better gel resolution.en_US
dc.identifier.citationArticale in Journal of fundamental and Applied Sciences Vol. 09, N. 02en_US
dc.identifier.issn1112-9867
dc.identifier.urihttp://dspace.univ-eloued.dz/handle/123456789/7427
dc.language.isoenen_US
dc.publisherUniversity of Eloued جامعة الواديen_US
dc.subjectFicus deltoidea; protein extraction; TCA-acetone; phenol-SDS; pH.en_US
dc.titleComparison Of Protein Extraction Methods For The Leaves Of Ficus Deltoideaen_US
dc.typeArticleen_US

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