دراسة الفاعلية البيولوجية والكيميائية لنبات النعناع

Abstract

Mentha spicata is a medical and aromatic plant of the oral family The study aimed to detect secondary metabolites as well as extract essential oil as well as to study biological and chemical effectiveness by testing DPPH root inhibition and FRAP retrograde capacity as well as quantitative estimation of polyphenol and flavonides. The results of the plant’s chemical detection tests examined showed the presence of alkaloids, tannins, saponates, flavonides, triplets and the absence of ratings. After the extraction process, the yield in the extract (ethanol _ water) is 0.92% and the base oil refund is 1.04%. By estimating the antioxidant activity of the extract (ethanol _ water) of the plant’s aerobic part using the DPPH test, the results showed that the IC50 value of the extract (ethanol _ water) was estimated at 134.67 ug/ml. Ascorbic acid was estimated at 60.25 ug/ml. In the FRAP retro-capability test the results showed that IC50 value of extract (ethanol _ water) was estimated at 193.78ug/ml for galic acid was estimated at 148.80 ug/ml. The quantitative estimate of many phenols and flavonides for the extract was estimated at 64.19 ug/ml and 97.76% respectively. Finally ,the study of antibacterial activity in order to determine the effectiveness of essential oil on certain Escherichia coli, Staphylococcus aureus ,Pseudomonas aeruginosa ,Klebsiella pneumoniae and by analyzing essential oil, we found that has activity against bacteria .