Browsing by Author "Tir Touil Meddah, A."

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    The Antibacterial Effect Of Two Medicinal Plants Inula Viscosa, Anacyclus Valentinus (asteraceae) And Their Synergistic Interaction With Antibiotic Drugs
    (University of Eloued جامعة الوادي, 2016-05-01) Side Larbi, K.; Meddah, B.; Tir Touil Meddah, A.; Sonnet, P.
    With the emergence of multidrug-resistant organisms, combining medicinal plants with synthetic medicines against resistant bacteria becomes necessary. In this study, Synergism between plants extracts (methanolic extract, essential oils) of Inula viscosa and Anacyclus valentinus and two commonly used antibiotics: gentamycin and oxacillin were investigated on three bacterian strains Escherichia. coli, Bacillus subtilis and Staphylococcus aureus. In the first time, the antibacterial effect of extracts alone was tested against 7 strains by disc diffusion and microdilution methods. The minimum inhibitory concentrations of methanolic extracts ranged between 6.25 and 50mg/ml while that of the essential oils varied between 12.5 and 100µL/mL. The inhibitory concentrations of antibiotics varied between 125 and 31.25 µg/ml. Interactions extracts /antibiotics and extracts/extracts were determined by disc diffusion agar and by checkboard. The results show that the synergistic effect of combinations plant extracts/antibiotics was more important than extracts/extracts.
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    Characterization Of Intestinal Microbiota In Celiac Children
    (University of Eloued جامعة الوادي, 2016-09-01) Lahcene, F.; Tir Touil Meddah, A.; Bouziane-nedjadi, K.; Meddah, B.; Leke, A.
    Celiac disease (CD) is enteropathy autoimmune induced by the ingestion of gluten in genetically predisposed subjects. The ingestion of gluten is responsible for the symptoms of CD, and a disturber of the intestinal microbiota. In this study, 13 Samples of intestinal biopsy, 15 fecal samples from celiac children, and 10 from controls children respectively were collected and analyzed by conventional culture technique to characterize the microbial profile intestinal of celiac children. There was 24 celiac children (8 boys), Mean age at diagnosis was 9.52 years, all have clinical manifestations, positive anti-transglutaminase antibodies and mucosal lesions suggestive of CD (Marsh Classification).We found a difference in intestinal microbiota, between celiac and controls children for example the Enterobacteria, Clostridium sp and Staphylococcus sp were remarkably higher in biopsy and fecal samples of celiac children than in controls. Inversely the Enterococcus sp, Lactobacillus sp and Clostridium sp were slightly lower in celiac children. Our results indicate an imbalance in intestinal microbiota for celiac children as reduction in the numbers of Lactobacillus sp, Enterococcus sp and increases in the numbers of Enterobacteria, Staphylococcus sp and Clostridium sp.