Browsing by Author "Sonnet, P."

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    Antibacterial And Antioxidant Activities Of The Essential Oils And Phenolic Extracts Of Myrtus Communis And Zygophylum Album From Algeria
    (University of Eloued جامعة الوادي, 2016-05-01) Belmimoun, A.; Meddah, B.; Meddah, A.t.; Sonnet, P.
    The present study deals with the evaluation of the in vitro antioxidant and antibacterial activity of phenolic extracts and essential oils of two medicinal and aromatic plants Zygophyllum album and Myrtus communis by using the 2,2- diphenyl-2-picrylhydrazyl radical ,total antioxidant power and agar diffusion methods and minimum inhibitory concentration (MIC) determination. Moreover, the extracts were investigated for their polyphenolic, flavonoids, tannins and anthocyans content by using the Folin-Ciocalteu assay, the aluminium trichlorid method, reaction with vanillin and colometer method based on differentiation of absorbance, respectively. The results showed that the highest antioxidant capacity was exhibited by the aqueous extract of Myrtus communis with IC50= 29,080mg/ml. All extracts possessed more or less antibacterial activity against the tested Gram- positive and Gram –negative bacteria Bacillus subtilis and E.coli were the more susceptible microorganisms to all extracts and essential oils. Pronounced antibacterial activity was observed by the methanolic extract of Zygophylum album (MIC value=25 µg/ml). Morever, the results showed that the phenolic compounds and flavonoids were abundant in Myrtle aqueous extracts.
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    The Antibacterial Effect Of Two Medicinal Plants Inula Viscosa, Anacyclus Valentinus (asteraceae) And Their Synergistic Interaction With Antibiotic Drugs
    (University of Eloued جامعة الوادي, 2016-05-01) Side Larbi, K.; Meddah, B.; Tir Touil Meddah, A.; Sonnet, P.
    With the emergence of multidrug-resistant organisms, combining medicinal plants with synthetic medicines against resistant bacteria becomes necessary. In this study, Synergism between plants extracts (methanolic extract, essential oils) of Inula viscosa and Anacyclus valentinus and two commonly used antibiotics: gentamycin and oxacillin were investigated on three bacterian strains Escherichia. coli, Bacillus subtilis and Staphylococcus aureus. In the first time, the antibacterial effect of extracts alone was tested against 7 strains by disc diffusion and microdilution methods. The minimum inhibitory concentrations of methanolic extracts ranged between 6.25 and 50mg/ml while that of the essential oils varied between 12.5 and 100µL/mL. The inhibitory concentrations of antibiotics varied between 125 and 31.25 µg/ml. Interactions extracts /antibiotics and extracts/extracts were determined by disc diffusion agar and by checkboard. The results show that the synergistic effect of combinations plant extracts/antibiotics was more important than extracts/extracts.