Browsing by Author "Rebiai, Abdelkrim"

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Chemical composition and antioxidant activity of Apis mellifera bee pollen from northwest Algeria
(Journal of Fundamental and Applied Sciences, 2012-12-31) Rebiai, Abdelkrim; Lanez, Touhami
The aim of this study was to assess the nutritional composition and antioxidant activity of bee pollen produced in the North western region of Algeria. The content of total phenolic compounds, flavonoids, and antioxidant activity using the molybdate ion reduction method were determined. The mean contents of phenolic compounds and total flavonoids were 30.46 ± 8.22 mg of GAE.g–1 pollen and 8.92 ± 5.5 mg of RE.g–1 pollen, respectively. High antioxidant activities were found for the molybdate ion reduction method, with values that ranged from 71.95 to 101.5 μg of GAE.g –1 pollen. The bee pollen of Boufarik showed high antioxidant activity probably due to the high content of phenolic compounds present in pollen.
Effect of Extraction Methods on Polyphenols, Flavonoids, Mineral Elements, and Biological Activities of Essential Oil and Extracts of Mentha pulegium L
(MDPI, 2021-12-21) Messaoudi, Mohammed; Rebiai, Abdelkrim; Sawicka, Barbara; Atanassova, Maria; Ouakouak, Hamza; Larkem, Imane; Egbuna, Chukwuebuka
ur study evaluated the in vitro antioxidant properties, antibacterial and antifungal activities, anti-inflammatory properties, and chemical composition of the essential oils (EOs), total phenol, and total flavonoid of wild Mentha pulegium L. This study also determined the mineral (nutritional and toxic) elements in the plant. The EOs were extracted using three techniques—hydro distillation (HD), steam distillation (SD), and microwave-assisted distillation (MAD)—and were analyzed using chromatography coupled with flame ionization (GC-FID) and gas chromatography attached with mass spectrometry detector (GC-MS). The antioxidant effects of the EOs were tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), while the antibacterial and antifungal activities of the EO and methanolic extract were tested using Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Twenty-six compounds were identified in the essential oil, representing 97.73% of the total oil, with 0.202% yield. The major components were pulegone (74.81%), menthone (13.01%) and piperitone (3.82%). Twenty-one elements, including macro- and micro-elements (Ba, Br, Ca, Cl, Co, Cr, Cs, Eu, Fe, K, Mg, Mn, Mo, Na, Rb, Sb, Sc, Sr, Th, U and Zn), were detected using neutron activation analysis (INAA) and inductively coupled plasma optical emission spectrometry (ICP-OES), with the concentration of mineral element close to the FAO recommendation. The results show that the EOs and extracts from Mentha pulegium L. had significant antimicrobial activities against the microorganisms, including five human pathogenic bacteria, one yeast (Candida albicans), and one phytopathogenic fungi. The in vivo anti-inflammatory activities of the leaf extracts were confirmed. The results indicate that the EOs and extracts from Mentha pulegium L. have promising applications in the pharmaceutical industries, clinical applications, and in medical research.
Effect of some agricultural residues on the characteristics of growth and yield of the onion bulb (Allium cepa L)
(University of eloued جامعة الوادي, 2019-04-15) Ghemam Amara, Djilani; Rebiai, Abdelkrim; Alia, Zaid; Djihad, Saoud; Kherraz, Khaled; Mesbahi, Mohammed Adel; Chemsa, Ahmed Elkhalifa; Senoussi, Mohammad Mourad
the study was conducted at the primary school farm in Rabbah zone during in 2016/2017 season, To evaluate the contribution of fram residues (palm residues, Peanut Peels, cooked tea leaves) in vegetative growth and the bulb onion in addition to the total yield of onion plant. An investigation was a randomized complete block design in three replications. The obtained results of experiment showed that: - Application of fertilize by cooked tea leaves were significantly better in of the studied characters (plants height and Leaves Area) with rate increased to 47.15% and 93.4% respectively, than control treatment. Followed by T1 and then T3. - Peanut Peels treatment gave the largest number of tubular blades in the plant(12.33), the diameter of the bulb neck(13.48mm) and bulb Length(7.35cm) than other treatments. Followed by T2 and then T3. -cooked tea leaves plants gave highest diameter of bulb(5.42cm), highest bulb weight(66g) and yield(20.85 t/ha) in the season. where Achieving with T1(18.35t/ha) significantly increase in the yield total in comparison with other treatments.
Effects Of Extraction Methods On Total Polyphenols, Free Radical Scavenging And Antibacterial Activity Of Crude Extracts Of Cleome Arabica L. Growing In Oued Souf Region
(جامعة الوادي - University of Eloued, 2020-08-31) Chouikh, Atef; Rebiai, Abdelkrim; Aref, Mahdia; Heded, Mounira; Adjal, El Hadda; Alia, Fatma
This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian). The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus.
Preparation methods keratin and nanoparticles keratin from wool: a review
(جامعة الوادي - university of el oued, 2020-07-05) Ben Seghir, , Bachir; Hemmami, Hadia; Zeghoud, Soumeia; Laouini, Salah Eddine; Rebiai, Abdelkrim; Ben Amor, Ilham; Souici, Imane; Beki, Ahlam
Keratins are a group of fibrous proteins, These proteins are abundantly present in nature and constitute the major part of the hair, wool, horns, nails, feathers, and the stratum corneum of the skin. Among others, an ecological and economic point of view, recovery of many organic substances discharged into the effluent of textile mills is of prime importance. This thesis review certain physical and chemical characteristics of the keratin derived utilizing five processes of chemical extraction: reduction, hydrolysis, sulfitolysis, oxidation, and production. Since the keratin is a group of fibrous protein and has many useful applications. For example: In pharmaceutical industries, plastic production, biosorption, and tissue engineering. Considering this advantage, this work mainly concentrates on the nanoparticle keratin synthesis through different methods
total polyphenol contents, radical scavenging and cyclic voltammetry of Algerian propolis
(International Journal of Pharmacy and Pharmaceutical Sciences, 2014) Lanez, Touhami; Rebiai, Abdelkrim; Belfar, M Lakhdar
Objective: In this study, we will determine the antioxidant properties of methanolic extract of propolis from Ghardaia and Khanchla provinces of Algeria and will correlate the values with total levels of polyphenolic compounds. Methods: The total polyphenol contents of methanolic extract of propolis were measured by using Folin–Ciocalteu spectrophotometric method. Thereafter, the antioxidant properties of these polyphenols were determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. All polyphenols extracted were tested using cyclic voltammetry (CV) in aqueous media. The CV was realised to compare the results from spectroscopic method and to electrochemically characterise the propolis polyphenols. Results: The total polyphenolic content in methanolic extract of propolis from Ghardaia and Khanchla was 493.49 and 1423.32 mg gallic acid equivalent/100g of extract, respectively. The IC50 values for scavenging DPPH radical for Ghardaia and Khanchla propolis were 0.03917 mg/mL, 0.01211mg/mL respectively. Antioxidant activity measured by cyclic voltammetry method indicated that methanolic extract of Khanchla propolis had AEAC of 15,61 mg/g. Conclusion: Propolis samples had strong antioxidant activities, and the highest activities were found in Khanchla propolis. Also, among three assays employed in this study (DPPH, RP, CV), the cyclic voltammetry method was recommended as it represented a relatively clean chemical system