Browsing by Author "Meddah, B."

Now showing 1 - 6 of 6

Antibacterial And Antioxidant Activities Of The Essential Oils And Phenolic Extracts Of Myrtus Communis And Zygophylum Album From Algeria
(University of Eloued جامعة الوادي, 2016-05-01) Belmimoun, A.; Meddah, B.; Meddah, A.t.; Sonnet, P.
The present study deals with the evaluation of the in vitro antioxidant and antibacterial activity of phenolic extracts and essential oils of two medicinal and aromatic plants Zygophyllum album and Myrtus communis by using the 2,2- diphenyl-2-picrylhydrazyl radical ,total antioxidant power and agar diffusion methods and minimum inhibitory concentration (MIC) determination. Moreover, the extracts were investigated for their polyphenolic, flavonoids, tannins and anthocyans content by using the Folin-Ciocalteu assay, the aluminium trichlorid method, reaction with vanillin and colometer method based on differentiation of absorbance, respectively. The results showed that the highest antioxidant capacity was exhibited by the aqueous extract of Myrtus communis with IC50= 29,080mg/ml. All extracts possessed more or less antibacterial activity against the tested Gram- positive and Gram –negative bacteria Bacillus subtilis and E.coli were the more susceptible microorganisms to all extracts and essential oils. Pronounced antibacterial activity was observed by the methanolic extract of Zygophylum album (MIC value=25 µg/ml). Morever, the results showed that the phenolic compounds and flavonoids were abundant in Myrtle aqueous extracts.
The Antibacterial Effect Of Two Medicinal Plants Inula Viscosa, Anacyclus Valentinus (asteraceae) And Their Synergistic Interaction With Antibiotic Drugs
(University of Eloued جامعة الوادي, 2016-05-01) Side Larbi, K.; Meddah, B.; Tir Touil Meddah, A.; Sonnet, P.
With the emergence of multidrug-resistant organisms, combining medicinal plants with synthetic medicines against resistant bacteria becomes necessary. In this study, Synergism between plants extracts (methanolic extract, essential oils) of Inula viscosa and Anacyclus valentinus and two commonly used antibiotics: gentamycin and oxacillin were investigated on three bacterian strains Escherichia. coli, Bacillus subtilis and Staphylococcus aureus. In the first time, the antibacterial effect of extracts alone was tested against 7 strains by disc diffusion and microdilution methods. The minimum inhibitory concentrations of methanolic extracts ranged between 6.25 and 50mg/ml while that of the essential oils varied between 12.5 and 100µL/mL. The inhibitory concentrations of antibiotics varied between 125 and 31.25 µg/ml. Interactions extracts /antibiotics and extracts/extracts were determined by disc diffusion agar and by checkboard. The results show that the synergistic effect of combinations plant extracts/antibiotics was more important than extracts/extracts.
Characterization Of Intestinal Microbiota In Celiac Children
(University of Eloued جامعة الوادي, 2016-09-01) Lahcene, F.; Tir Touil Meddah, A.; Bouziane-nedjadi, K.; Meddah, B.; Leke, A.
Celiac disease (CD) is enteropathy autoimmune induced by the ingestion of gluten in genetically predisposed subjects. The ingestion of gluten is responsible for the symptoms of CD, and a disturber of the intestinal microbiota. In this study, 13 Samples of intestinal biopsy, 15 fecal samples from celiac children, and 10 from controls children respectively were collected and analyzed by conventional culture technique to characterize the microbial profile intestinal of celiac children. There was 24 celiac children (8 boys), Mean age at diagnosis was 9.52 years, all have clinical manifestations, positive anti-transglutaminase antibodies and mucosal lesions suggestive of CD (Marsh Classification).We found a difference in intestinal microbiota, between celiac and controls children for example the Enterobacteria, Clostridium sp and Staphylococcus sp were remarkably higher in biopsy and fecal samples of celiac children than in controls. Inversely the Enterococcus sp, Lactobacillus sp and Clostridium sp were slightly lower in celiac children. Our results indicate an imbalance in intestinal microbiota for celiac children as reduction in the numbers of Lactobacillus sp, Enterococcus sp and increases in the numbers of Enterobacteria, Staphylococcus sp and Clostridium sp.
EFFECT OF SALSOLA VERMICULATA ON FUNGI OF FRENCH SOFT WHEAT AND TEST OF GRAIN STORAGE BY THE COATING METHOD
(University of Eloued جامعة الوادي, 2018-01-01) Moghtet, S.; Menad, N.; Meddah, B.; Moussaoui, A.
In the Algerian pharmacopoeia, The search for medicinal plants is an objective, Salsola vermiculata is one of the most traditional plant remedies. in this work, we tried to test the inhibitory effect on eight storage soft wheat fungi of various extracts of Salsola vermiculata in vitro and vivo by using grain storage by the coating method.The phytochemical screening of plants showed the detection of tannins, saponisides and flavonoids.The results show a remarkable antifungal effect by most of the extracts tested. The decrease in the level of fungi observed in wheat samples demonstrates the effectiveness of grain storage by the coating method.
Removal Of Zinc And Cadmium Ions From Contaminated Soils With Rhamnolipid Biosurfactant Produced By Pseudomonas Aeruginosa S7ps5
(University of Eloued جامعة الوادي, 2016-09-01) Bendaha, M. E-a.; Meddah, B.; Belaouni, H. A.; Mokhtar, M.; Tirtouil, A.
A soil treatment process using froth flotation technique involving anionic biosurfactant (rhamnolipids) using Sodium sulfide was studied. The supernatant produced by the strain Pseudomonas aeruginosa S7PS5 was tested for biosurfactants production, HPLC analysis showed the presence of L-rhamnosyl- β- hydroxydecanoyl- β-hydroxydecanoate (RL1) and L-rhamnosyl L-rhamnosyl- β-hydroxydecanoyl-β- hydroxydecanoate (RL2). The influence of the collector (rhamnolipid), pulp pH, a chemical activation step (sulfidization) and process time on metal removal efficiency has been investigated to recover Zn and Cd ions from a contaminated soil. An effective CMC of 35 mg/L was obtained. A perfect Zn and Cd removal efficiency was made at pH = 12 and 4 mg/g of Na2S during the first 5 min of soil washing process, then a longer flotation time ( > 5 min) caused mechanical entrainment of Zn and Cd.
THE USE OF ORANGE (CITRUS SINENSIS) PEEL AS ANTIMICROBIAL AND ANTI-OXIDANT AGENTS
(University of Eloued جامعة الوادي, 2017-09-01) Ibri, K.; Bouhadi, D.; Hariri, A.; Meddah, B.; Tir Touil, A.; Ould Yerou, K.
Due to rapid growth of the food processing industry and the consumption of processed foods, the demand for natural antimicrobial agents is on the rise. Consumers have become more aware about the health effects of the synthetic preservatives used in food. Hence natural preservatives are developed to meet the demand of consumers. These natural antimicrobials are developed either from plants or their parts, animals or even microorganisms. Even the waste generated from the food industries is being considered as an alternative to produce natural antimicrobials. The aim of the present study was to utilize the waste generated from the citrus fruit processing (peel) industry. This study was to utilize the powder from the peel of Citrus sinensis in food (Oil of olive and cream dessert) to preserve their quality, this peel can be used as antimicrobial and antioxidant activity so food preservation purpose.