Browsing by Author "Lanez, Touhami"

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    Chemical composition and antioxidant activity of Apis mellifera bee pollen from northwest Algeria
    (Journal of Fundamental and Applied Sciences, 2012-12-31) Rebiai, Abdelkrim; Lanez, Touhami
    The aim of this study was to assess the nutritional composition and antioxidant activity of bee pollen produced in the North western region of Algeria. The content of total phenolic compounds, flavonoids, and antioxidant activity using the molybdate ion reduction method were determined. The mean contents of phenolic compounds and total flavonoids were 30.46 ± 8.22 mg of GAE.g–1 pollen and 8.92 ± 5.5 mg of RE.g–1 pollen, respectively. High antioxidant activities were found for the molybdate ion reduction method, with values that ranged from 71.95 to 101.5 μg of GAE.g –1 pollen. The bee pollen of Boufarik showed high antioxidant activity probably due to the high content of phenolic compounds present in pollen.
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    total polyphenol contents, radical scavenging and cyclic voltammetry of Algerian propolis
    (International Journal of Pharmacy and Pharmaceutical Sciences, 2014) Lanez, Touhami; Rebiai, Abdelkrim; Belfar, M Lakhdar
    Objective: In this study, we will determine the antioxidant properties of methanolic extract of propolis from Ghardaia and Khanchla provinces of Algeria and will correlate the values with total levels of polyphenolic compounds. Methods: The total polyphenol contents of methanolic extract of propolis were measured by using Folin–Ciocalteu spectrophotometric method. Thereafter, the antioxidant properties of these polyphenols were determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. All polyphenols extracted were tested using cyclic voltammetry (CV) in aqueous media. The CV was realised to compare the results from spectroscopic method and to electrochemically characterise the propolis polyphenols. Results: The total polyphenolic content in methanolic extract of propolis from Ghardaia and Khanchla was 493.49 and 1423.32 mg gallic acid equivalent/100g of extract, respectively. The IC50 values for scavenging DPPH radical for Ghardaia and Khanchla propolis were 0.03917 mg/mL, 0.01211mg/mL respectively. Antioxidant activity measured by cyclic voltammetry method indicated that methanolic extract of Khanchla propolis had AEAC of 15,61 mg/g. Conclusion: Propolis samples had strong antioxidant activities, and the highest activities were found in Khanchla propolis. Also, among three assays employed in this study (DPPH, RP, CV), the cyclic voltammetry method was recommended as it represented a relatively clean chemical system