Browsing by Author "Ben Ayed, R."

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Comparison Between Dna-based, Pomological And Chemical Markers Accomplished By Bioinformatic Tools To Distinguish Within Tunisian Olive Cultivars
(University of Eloued جامعة الوادي, 2015-09-01) Ben Ayed, R.; Ennouri, K.; Ben Hassen, H.; Triki, M. A.; Rebai, A.
The genetic diversity of 16 Tunisian olive cultivars (Olea europaea L.) of known origin sampled from different areas of the country was assessed using genetic markers (6 SSR and 5 SNP markers). Three dendrograms based on cultivar genotypes generated by SSR, SNP and both SSR and SNP markers revealed three clusters which were consistent with the varieties classification according to phenotypic characteristics, but not correlated with the geographic origin. Also, we compared the results obtained with the genetic markers to those obtained with agro-morphological and chemicals data using bioinformatic analyses. This work provides better understanding of the diversity available in Tunisia olive cultivars and supplies an important contribution for olive breeding and olive oil authenticity.
INVOLVEMENT OF SNP MARKER LOCATED ON THE CALCIUM BINDING PROTEIN GENE IN ADAPTIVE TRAITS AND ORGANOLEPTIC PERFORMANCES OF THE OLIVE TREE.
(University of Eloued جامعة الوادي, 2018-01-01) Ben Ayed, R.; Ennouri, K.; Rebai, A.
Olive trees importance is mainly due to the economic and health benefits, especially in the Mediterranean basin. Unfortunately, to enhance productivity and quality of olive oil, the study of both molecular and phenotypic characterizations of olive cultivars is crucial. We consider the analysis of 14 Tunisian olive cultivars of known origin sampled from different areas of the country based on SNP marker (CALC) located in calcium binding protein gene. Accordingly, we assessed the potential genotype-phenotypes links between the SNP, on the one hand, and the quantitative and qualitative parameters, on the other. The statistical analysis based on bivariate analysis for the CALC marker suggests that Chemlali varieties with CG genotypes have better capacity to produce fruit, fruit oil, oil content in carotene, cholesterol and linoleic acid than homozygote GG genotypes varieties. These results are confirmed by a multivariate logistic regression.
MOLECULAR CHARACTERIZATION OF Î“ GLIADIN FROM DURUM WHEAT (TRITICUM TURGIDUM L. SUBSP. DURUM ((DESF.) HUSN.)
(University of Eloued جامعة الوادي, 2017-09-01) Mzid, R.; Ayadi, M.; Ben Ayed, R.; Menzli, E.; Babay, E.; Hanana, M.
The gluten quality is one of the main factors affecting the quality of durum wheat. It depends primarily on its storage proteins composition (glutenins and gliadins). In order to set up and initiate a technological quality improvement program of durum wheat we have conducted a prospection of the different protein sequences of gliadin in different databases for Triticum, then the filtration steps and assembly by appropriate software have been conducted to reduce the number of redundant sequences. On the other hand, we have isolated a gene from Iride "Gli-A1" encoding a Î³-gliadin protein associated with gluten strength and viscoelasticity of the dough, we performed an in silico molecular and structural analysis in order to define its putative functional properties. The latter could be a valuable candidate as molecular marker for selecting high nutritive value of durum wheat and/or for genetic improvement of durum wheat quality.