المساھمة في دراسة نواتج الأیض الثانوي و الفعالیة البیولوجیة لمستخلصات نبات القزاح : Pituranthos chloranthus
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Date
2019
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Publisher
Université of Eloued
Abstract
This study aimed to investigate the outputs of secondary metabolism and determine the
biological effectiveness of Pituranthos Chloranthus' extracts.
In order to achieve this goal, we first used the chemical examination, which showed the
presence of most secondary metabolites. next, Preparation of methanol, ethanol and aqueous
extracts by soaking and the extraction of essential oil through hydro-distillation.
In turn, the yield of each extract has been recorded; the highest percentage has been
associated with the methanolic extract at 5%, followed by 4.5% that of the aqueous extract,
and the ethanolic extract by 3.46%, the yield recorded in essential oil was 0.4%. The plant's
moisture content was estimated at 43.8%.
Secondly, phenol, flavonoids and tannins were estimated in aqueous, methanolic and
ethanolic extracts. The highest poly phenol content was found in the methanolic extract
(14.65± 2.33 mg AG E/g MS), while the lowest value spotted in aqueous extract (11.22±0.98
mg AG E/g MS). The quantative estimate of flavonoids was as its highest value in methanolic
extract at an estimate of (2.9885±0.035 mg Q E/g MS) and the lowest value was found in
aqueous extract (0.7494±0.013 mg Q E/g MS) , whereas the highest value concerning tannins
was spotted in ethanolic extract with an estimate of (5.31±0.16 mg CA E/g MS) and its lowest
value in aqueous extract (4.04±0.30mg CA E/g MS)
Thirdly, in terms of biological efficiency, the aqueous, methanolic and ethanolic extracts
showed a moderate antioxidant activity where the IC50 was estimated at 0.54-0.57-0.59 mg/ml
respectively, while the essential oil antioxidant activity was low and IC50 estimated to be
4.49 mg/ml .
the antibacterial activity for four bacterial strains (Escherichia coli, Pseudomonas aeruginosa,
Staphylococcus aureus, Bacillus cereus) was positive only with the pure essential oil and was
not with the rest of the extracts, and the best diameter inhibition was recorded at the 100%
concentration with the value of 14.9 mm in S. aureus .
Description
Biologie et Valorisation des Plantes
Keywords
نبات القزاح, Pituranthos chloranthus