Ghouila, Z.Laurent, S.Boutry, S.Vander Elst, L.Nateche, F.Muller, R. N.Baaliouamer, A.2020-11-022020-11-022017-01-01Article in Journal of Fundamental and Applied Sciences Vol 09, N 011112-9867http://dspace.univ-eloued.dz/handle/123456789/7376Article in Journal of Fundamental and Applied Sciences Vol 09, N 01In this work and for the first time, significant concentrations of total polyphenols and flavonoids from Vitis vinifera L. grape seed extracts were obtained (256.15 ± 17.40 mg GAE/gdm and 14.08 ± 0.64 mg CE/gdm, respectively).The LC/MS analysis revealed richness in procyanidins. For antioxidant, antimicrobial and antifungal effects, the grape seed extract (GSE) responded positively. At 100 μg/mL, GSE induced a moderate toxicity of the order of 3.88% on 3T6 cells at the first 24 hours of treatment, whereas, its prolonged effect to 48 hours reduced this toxicity to less than 0.5%. As for the anti-proliferative effect on tumoral cell lines, a cell death of 18.39% to 23.79% and 10.30% to 20.37% was registered respectively for HeLa and BCPAP cells during 24 and 48 hours of treatment. Consequently, it is possible to consider using GSE at lower concentrations as an anti-proliferative agent without losing sight of its benefic effect on healthy cells.engrape seed extract, 3T6 cell, antioxidant, antibacterial, anti-proliferation.Article