Khaled, Sobhi*,Souhila r, Mélaïne,Mohamed, Mammar2023-05-312023-05-312022-05-01Khaled Sobhi*, Souhila Mélaïne, Abderrahmane Abdaoui, Mohamed Mammar. HPLCTOXICOLOGICAL SCREENING METHOD BY HPLC TOXICOLOGICAL SCREENING METHOD BY HPLC TOXICOLOGICAL SCREENING METHOD BY HPLC TOXICOLOGICAL SCREENING METHOD BY HPLCTOXICOLOGICAL SCREENING METHOD BY HPLC TOXICOLOGICAL SCREENING METHOD BY HPLC TOXICOLOGICAL SCREENING METHOD BY HPLCTOXICOLOGICAL SCREENING METHOD BY HPLCTOXICOLOGICAL SCREENING METHOD BY HPLC TOXICOLOGICAL. Journal of Fundamental and sciences. vol.14, no 2. May 2022. Faculty of exact sciences. university of el oued. [visited in 01/12/2021. available from [copy the link here]http://dspace.univ-eloued.dz/handle/123456789/25181articalA liquid-liquid extraction technique and HPLC-DAD method using a C18 column, 5μm (150x4.6mm i.d.) were developed for a toxicological drug screening. A home-made “N-acetylparoxetine” is used as internal standard. A local UV spectra library of over 130 drugs and some of their metabolites was created. This library covers the main therapeutic classes involved in intoxication cases. The developed procedure is successfully applied to different biological samples (serum, urine, gastric fluid and post mortem whole blood) and allows rapid and simultaneous detection and identification of a large number of substances and their semi-quantification in serum.enHPLC-DAD, LLE, drug poisoning, systematic toxicological analysis.Article