ABBAS IsmahaneROUAG Ouarda2025-06-302025-06-302025master, 2025. DEPARTEMENT DE BIOLOGIE CELLULAIRE ET MOLECULAIRE. Faculté des Sciences de la Nature et de la Vie. Université d'El-Oued.https://dspace.univ-eloued.dz/handle/123456789/38397Our study aimed to evaluate the therapeutic effect of dromedary fat-capped zinc nanoparticles (DF-ZnNPs) on oxidative stress, immunosuppression, and biochemical disturbances induced by cisplatin (an anticancer chemotherapeutic drug) in rats. To investigate the biosynthesis of dromedary camel fat-coated zinc nanoparticles, ultraviolet-visible (UV-VIS), Fourier Transform Infrared spectroscopy (FTIR), scanning electron microscopy (SEM), EDX microscopy, transmission electron microscopy (TEM), and gas chromatography-mass spectrometry (GC-MS) were used and an antibacterial activity of these elements was tested. In an in-vivo study 26 male albino wistar rats,6 rats for toxicity testing and 20 rats were randomly divided into four groups (five in each): Control group, Cisplatin group (CP), rats treated with CP and DF-ZnNPs group (CP+ DF-ZnNPs) and rats treated with CP and dromedary fat group (CP+DF). CP induced toxicity using intraperitoneally single dose 5mg/kg. DF-ZnNPs and DF treated intraperitoneally at a dose 5 mg/kg/week in each. Various hematological, biochemical, and oxidative stress markers were estimated. Histopathology of liver, lungs and kidneys tissues was observed. The results were as expected for the cisplatin group, with mice showing different results compared to the control group. Fluctuations occurred in biochemical parameters (blood sugar p**<0.01, cholesterol. Triglycerides (p**<0.01, cretin p*<0.05, TGO p*<0.05, TGP p**<0.01) and hematological parameters (RBC, HGB, HCT, MCV p***<0.001, MCH. PLT p***<0.01, WBC, lymphocytes, granulocytes (p***<0.001), as well as oxidative stress in liver, kidney, lung, and heart tissues. As for the treatment regimens, they showed a positive effect in improving biomarkers, reducing oxidative stress, and histological analyses, demonstrating the effectiveness of the nanoparticles. In conclusion, the observed improvement in the studied parameters encourages the use of the DF ZnNPs as a new treatment accompanying chemotherapy to reduce the symptoms resulting from its secondary effects. Therefore, we need further in-depth studies at the cellular and molecular level to confirm the efficacy of zinc and to ensure the safety of its users from any harm that may occur.enCisplatinDF-ZnNPsdromedary fatOxidative stressLiverkidneylungmaster