Boukhatem, M. N.Ferhat, M. A.Hadj Mohamed, R.Lalaoui, N.2020-10-212020-10-212015-05-01Articale in Journal of fundamental and Applied Sciences Vol. 07, N. 021112-9867http://dspace.univ-eloued.dz/handle/123456789/7241Articale in Journal of fundamental and Applied Sciences Vol. 07 N. 02The resurgence of multiple antibiotic resistances is a major public health problem, often causing therapeutic impasses. Our study was conducted at the Bacteriology Laboratory of Kolea (Tipaza, Algeria) Hospital. The aim of this study was to determine the prevalence of staphylococci in clinical purulent, sepsis and urinary samples, and to test the sensitivity of the selected strains against a various antibiotic (ATB) discs. The identification of Staphylococcus strains was performed by using classic biochemistry tests. Susceptibility against ATB was determined by the agar disk diffusion method. Among 1403 clinical samples obtained, only 61 was related to staphylococci infections (4.34%). Staphylococcus strains were found mainly in the purulent samples (60.65%). S.aureus was found to be the mainly isolated strains with a rate of 61.53% while the S. epidermidis strain was found predominantly in hospitalized patients (68.57%). The frequency of the overall resistance of S.aureus against penicillin G (80.6%) and gentamicin (61.53%) remains high. The multi-resistant Staphylococcus has become extremely widespread, hence the need for the accomplishment of a strategy to prevent the spread of this resistance.frStaphylococci; Staphylococcus aureus; Oxacillin; Antibiotic resistance; Disc diffusion.Article